Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Reexamination Certificate
2007-07-31
2007-07-31
Swope, Sheridan (Department: 1652)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
C435S252300, C435S320100, C435S194000, C435S069700, C435S071100, C536S023200
Reexamination Certificate
active
10890675
ABSTRACT:
The present invention relates to a functional assay for IκB kinase (IKK), the proteasome and ubiquitin ligase. Cells expressing an IκB-beta-lactamase fusion protein are used to screen for inhibitors of IKK, the proteasome and ubiquitin ligase. Inhibitors identified through the inventive assay are useful in the treatment of NF-κB disorders, such as immune and inflammatory disorders. The present invention also includes cell lines useful in assays of the invention, compounds identified by assays of the invention, compositions including such compounds and methods for the treatment of disease states.
REFERENCES:
Galye et al, Identification of regions in interleukin-1 alpha important for activity. J Biol Chem. Oct. 15, 1993;268(29):22105-11.
Whisstock et al, Prediction of protein function from protein sequence and structure. Q Rev Biophys. Aug. 2003;36(3):307-40. Review.
PIR—80 database Accession No. A39935 Jan. 17, 1992 Haskill et al. Alignment with SEQ ID No. 1.
Haskill et al, Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity. Cell. Jun. 28, 1991;65(7):1281-9.
Whitney et al, A collaborative screening program for the discovery of inhibitors of HCV NS2/3 cis-cleaving protease activity. J Biomol Screen. Apr. 2002;7(2):149-54.
Zamanian-Daryoush et al, NF-kappaB activation by double-stranded-RNA-activated protein kinase (PKR) is mediated through NF-kappaB-inducing kinase and IkappaB kinase. Mol Cell Biol. Feb. 2000;20(4):1278-90.
Karin et al, Phosphorylation meets ubiquitination: the control of NF-[kappa]B activity. Annu Rev Immunol. 2000;18:621-63. Review.
Burke, James R., “BMS-345541 Is a Highly Selective Inhibitor of IkB Kinase That Binds at an Allosteric Site of the Enzyme and Blocks NF-kB-dependent Transcription in Mice”, The Journal of Biological Chemistry, vol. 278, No. 3, Issue of Jan. 17, 2003, pp. 1450-1456.
Burke James R.
Wang Shulin
Bristol--Myers Squibb Company
Carey Brian C.
Swope Sheridan
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