Assay for measuring gene expression

Details Assay for measuring gene expression Assay for measuring gene expression

1985-03-15

1987-04-28

Martinell, James

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 68, 435 70, 435 91, 436 63, 436 94, 935 44, 935 79, C12Q 168, C12P 2100, C12P 2102, C12P 1934, G01N 3348, G01N 3300

Patent

active

046614435

ABSTRACT:
A DNA-directed in vitro assay for measuring the formation of the initial NH.sub.2 -terminal dipeptides or tripeptides which are characteristic of a particular gene product is disclosed. This invention also encompasses a kit consisting of the reagents necessary to perform this method so as to measure or identify a product of gene expression.

REFERENCES:
Cenatiempo et al: "Use of Different tRNA.sup.Ser Isoacceptor Species in Vitro to Discriminate Between the Expression of Plasmid Genes", Proc. Natl. Acad. Sci. USA 79: 1466 (1982).
Robakis et al, "Translational Control of Ribosomal Protein L10 Synthesis Occurs Prior to Formation of First Peptide Bond", Proc. Natl. Acad. Sci. USA 78: 4261 (1981).
Goldberg et al, "In Vitro Regulation of DNA-Dependent Synthesis of Escherichi coli Ribosomal Protein L12", Proc. Natl. Acad. Sci. USA 76: 1716 (1979).
Modolell, "The Initial Steps in Protein Synthesis: Effects by Antibiotics", Methods in Enzymology, vol. 30, Part F, pp. 79-86 (1974).

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