Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-11-10
2010-11-23
Woolwine, Samuel (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C536S023100, C435S183000
Reexamination Certificate
active
07838230
ABSTRACT:
Provided are methods, such as high throughput methods, of assessing or measuring the ability of an enzyme to modify the topology (e.g. supercoil topology) of a target nucleic acid, the methods comprising the steps of: (a) providing a solid support to which a capture nucleic acid is or may be immobilized, which capture nucleic acid is capable of binding the target nucleic acid in a manner that is proportional to the supercoil topology of said target nucleic acid (e.g. by triplex formation); (b) incubating a test mixture comprising (i) the enzyme, (ii) the target nucleic acid, (iii) capture nucleic acid, in the presence of (iv) said solid support, such that supercoiled target nucleic acid bound by the capture nucleic acid is immobilized to the solid support, (c) determining the amount of target nucleic acid bound by said capture nucleic acid in step (b) e.g. by use of a detectable label. The method may be used to screen for modulators of the enzyme activity.
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Plant Bioscience Limited
Sleath Janet
Speckman Law Group PLLC
Woolwine Samuel
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