Assay for ligands by separating bound and free tracer from sampl

Details Assay for ligands by separating bound and free tracer from sampl Assay for ligands by separating bound and free tracer from sampl

1984-02-23

1989-09-12

Hill, Jr., Robert J.

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

Separation of immune complex from unbound antigen or antibody

436538, 436546, 436548, 436824, G01N 33538

Patent

active

048659975

ABSTRACT:
In an assay wherein there is produced in an assay sample tracer bound to a soluble binder and unbound tracer, both bound and unbound tracer are separated from the assay sample for separate determination thereof by use of a supported binder for the soluble binder and a supported binder for the tracer. The assay may be effected as a "flow-through assay" by causing the sample to flow through successive chambers containing the supported binders.

REFERENCES:
patent: 4022577 (1977-05-01), Brooker et al.
patent: 4268494 (1981-05-01), Reese
patent: 4376110 (1983-03-01), David

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