Assay for inositol phosphorylceramide synthase activity

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase

Reexamination Certificate

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C435S194000, C436S172000

Reexamination Certificate

active

07659088

ABSTRACT:
Disclosed is a simple and reproducible method for assaying inositol phosphorylceramide synthase activity that employs a fluorescence resonance energy transfer pair for measuring enzyme activity. The invention also includes a novel method for identifying IPC synthase inhibitors.

REFERENCES:
patent: 5976866 (1999-11-01), Heidler et al.
patent: 6022684 (2000-02-01), Radding et al.
patent: 6197928 (2001-03-01), Tsien et al.
patent: 6596499 (2003-07-01), Jalink
patent: 6808892 (2004-10-01), Schnell et al.
patent: 7105636 (2006-09-01), Wrana
patent: 2005/0048563 (2005-03-01), Jalink
patent: 2006/0112440 (2006-05-01), Tsien et al.
patent: 2007/0111270 (2007-05-01), Zhang et al.
patent: WO-98/46639 (1998-10-01), None
patent: WO 00/18949 (2000-04-01), None
Aeed, Paul A., et al, Effect of Membrane Perturbants on the Activity and Phase Distribution of Inositol Phophorylceramide Synthase; Development of a Novel Assay,Biochemistry2004, 43, 8483-8493, American Chemical Society.
Figueiredo, Juliana M., et al, Characterization of the inositol phosphorylceramide synthase activity fromTrypanosoma cruzi, Biochem. J.(2005) 387, 519-529.
Fischl, Anthony S., et al, Inositolphosphoryl Ceramide Synthase from Yeast,Methods In Enzymology, vol. 311, 123-130, Academic Press.
Mandala, Suzanne M., et al, Khafrefungin, a Novel Inhibitor of Sphingolipid Synthesis,The Journal of Biological Chemistry, vol. 272, No. 51, Issue of Dec. 19, pp. 32709-32714, 1997, The American Society for Biochemistry and Molecular Biology, Inc.
Mandala, Suzanne M., et al, Rustmicin, a potent Antifungal Agent, Inhibits Sphingolipid Synthesis at Inositol Phosphoceramide Synthase,The Journal of Biological Chemistry, vol. 273, No. 24, Issue of Jun. 12, pp. 14942-14949, 1998, The American Society of Biochemistry and Molecular Biology, Inc.
Nagiec, M. Marek, et al, Sphingolipid Synthesis as a Target for Antifungal Drugs,The Journal of Biological Chemistry, vol. 272, No. 15, Issue of Apr. 11, pp. 9809-9817, 1997, The American Society for Biochemistry and Bolecular Biology, Inc.
Zhong, Wenyan, et al, Inhibition of yeast inositol phosphorylceramide synthase by aureobasidin, a measured by a fluorometric assay,FEBS Letters463 (1999) 241-244, Federation of European Biochemical Societies.

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