Assay for identifying compounds which affect stability of mRNA

Details Assay for identifying compounds which affect stability of mRNA Assay for identifying compounds which affect stability of mRNA

2004-04-01

2009-10-06

Crouch, Deborah (Department: 1632)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

C435S007100, C435S029000

Reexamination Certificate

active

07598079

ABSTRACT:
The present invention relates to an assay for the identification of biologically active compounds, in particular to a reporter gene assay for the identification of compounds, which have an effect on mRNA stability. More particularly, the present invention relates to a reporter gene expression system and cell lines comprising said expression system. The invention further relates to compounds which destabilize mRNA.

REFERENCES:
patent: 5444149 (1995-08-01), Keene et al.
patent: 5587300 (1996-12-01), Malter
patent: 5698427 (1997-12-01), Keene et al.
patent: 5731343 (1998-03-01), Feng et al.
patent: 6635671 (2003-10-01), Kastelic et al.
patent: 7078171 (2006-07-01), Giordano et al.
patent: 2004/0214223 (2004-10-01), Cao et al.
patent: 2005/0048549 (2005-03-01), Cao et al.
patent: 2007/0190532 (2007-08-01), Katelic et al.
patent: 9828707.1 (1999-02-01), None
patent: 9828710.5 (1999-02-01), None
patent: WO-93/20212 (1993-10-01), None
patent: WO-95/33831 (1995-12-01), None
patent: WO-98/39484 (1998-09-01), None
patent: WO-99/61605 (1999-12-01), None
patent: WO-99/61605 (1999-12-01), None
patent: WO-99/61605 (1999-12-01), None
patent: WO-00/38674 (2000-07-01), None
patent: WO-00/39314 (2000-07-01), None
patent: WO-02/072844 (2002-09-01), None
patent: WO-2004/065561 (2004-08-01), None
Lemm and Ross (Molecular and Cellular Biology, 2002, vol. 22, No. 12, pp. 3959-3969).
Banholzer et al (Molecular Cellular Biology, 1997, vol. 17, No. 6, pp. 3254-3260).
Zubiaga et al. (1995, MCB, vol. 15, No. 4, pp. 2219-2230).
Kastelic et al (Cytokine, 1996. vol. 8, No. 10, pp. 751-761).
Levy et al (JBC, 1996, vol. 271, No. 5, pp. 2746-2753).
Rajagopalan et al (Journal of Neurochem. 2000. vol. 74, pp. 52-59).
Capaccioli et al. (Oncogene, 1996, vol. 13, pp. 106-115).
Yeilding et al (Molecular and Cellular Biology, 1996.vol. 16, No. 7, pp. 3511-3522).
Zhang et al (Biochemical and Biophysical Research Communications, 1996. vol. 227, No. 3, pp. 707-711).
Merriam Webster Online dictionary definition of “derive.”, (2007).
Akashi, M. et al. (1994). “Number and Location of AUUUA Motifs: Role in Regulating Transiently Expressed RNAs,”Blood83:3182-3187.
Auwerx, J. (1991). “The Human Leukemia Cell Line, THP-1: A Multifaceted Model for the Study of Monocyte-Macrophage Differentiation,”Experienta47:22-31.
Banholzer, R. et al. (Jun. 1997). “Rapamycin Destabilizes Interleukin-3 mRNA in Autocrine Tumor Cells by a Mechanism Requiring an Intact 3′ Untranslated Region,”Molecular and Cellular Biology17(6):3254-3260.
Bernstein, P.L. et al. (Apr. 1992). “Control of c-mycmRNA Half-Life in vitro by a Protein Capable of Binding to a Coding Region Stability Determinant,”Genes Dev. 6(4):642-654.
Beutler, B. et al. (1988). “Assay of a Ribonuclease That Preferentially Hydrolyses mRNAs Containing Cytokine-Derived UA-Rich Instability Sequences,”Biochem. Biophys. Res. Comm. 152:973-980.
Chen, C.-Y.A. et al. (1994). “Interplay of Two Functionally and Structurally Distinct Domains of the c-fosAU-Rich Element Specifies Its mRNA-Destabilizing Function,”Mol. Cell. Biol. 14:416-426.
Chen, C.-Y.A. et al. (1994). “Selective Degradation of Early-REsponse-Gene mRNAs: Functional Analyses of Sequence Features of the AU-Rich Elements,”Mol. Cell. Biol. 14:8471-8482.
Chen, C.-Y.A. et al. (1995). “AU-Rich Elements: Characterizatiopn and Importance in mRNA Degradation,”TIBS20:465-470.
Chen, C.-Y.A. et al. (1995). “mRNA Decay Mediated by Two Distinct AU-Rich Elements from c-fosand Granulocyte-Macrophage Colony-Stimulating Factor Transcripts: Different Deadenylation Kinetics and Uncoupling from Translation,”Mol. Cell. Biol. 15:5777-5788.
Claffey, K.P. et al. (Feb. 1998). “Identification of a Human VPF/VEGF 3′ Untranslated Region Mediating Hypoxia-Induced mRNA Stability,”Mol. Biol. Cell9:469-481.
Cleveland, D.W. et al. (Nov. 1989). “Multiple Determinants of Eukaryotic mRNA Stability,”New. Biol. 1(2):121-126.
Crawford, E.K. et al. (Aug. 1997). “The Role of 3′ Poly(A) Tail Metabolism in Tumor Necrosis Factor-α Regulation,”J. Biol. Chem. 272:21120-21127.
Danner, S. et al. (Feb. 1998). “Agonist REgulation of Human β2-Adrenergic Receptor mRNA Stability Occurs via a Specific AU-Rich Element,”J. Biol. Chem.273:3223-3229.
Fan, X.C. (Jun. 1998). “Overexpression of HuR, a Nuclear-Cytoplasmic Shuttling Protein, Increases the in vivo stability of ARE-Containing mRNAs,”EMBO J. 17:3448-3460.
GenBank Accession No. AF022375, created Oct. 7, 1998, located at <http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=3719220>, last visited on Feb. 7, 2007, two pages.
GenBank Accession No. D10493, created May 29, 2002, located at <http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&val=219932>, last visited on Feb. 7, 2007, seven pages.
GenBank Accession No. M13994, created Oct. 31, 1994, located at <http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=179366>, last visited on Dec. 29, 2006, three pages.
GenBank Accession No. U40398, created Mar. 13, 1997, located at <http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=1117909>, last visited on Dec. 29, 2006, three pages.
GenBank Accession No. X04500, created Nov. 14, 2006, located at <http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=33788>, last visited on Dec. 29, 2006, six pages.
GenBank Accession No. Y00264, created Sep. 12, 1993, located at <http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=28525>, last visited on Dec. 29, 2006, three pages.
Gil, P. et al. (1996). “Multiple Regions of theArabidopsis SAUR-AC1Gene Control Transcript Abundance: the 3′ Untranslated Region Functions as a mRNA Instability Determinant,”EMBO J. 15:1678-1686.
Heaton, J.H. et al. (Jun. 1998). “Cyclic Nucleotide Regulation of Type-1 Plasminogen Activator-Inhibitor mRNA Stability in Rat Hepatoma Cells,”J. Biol. Chem. 273:14261-14268.
International Search Report mailed Jun. 6, 2000, for PCT Application No. PCT/CA99/01235 filed Dec. 23, 1999, three pages.
Kastelic, T. et al. (Oct. 1996). “Induction of Rapid IL-1B mRNA Degradation in Thp-1 Cells Mediated Through the AU-rich Region in the 3′ UTR by a Radcicol Analogue,”Cytokine8(10):751-761.
Klausner, R.D. et al. (1993). “Regulating the Fate of mRNA: The Control of Cellular Iron Metabolism,”Cell72:19-28.
Kobayashi, M. et al. (Jul. 1998). “Characterization of the 3′ Untranslated Region of Mouse DNA Topoisomerase Ilα mRNA,”Gene215:329-337.
Lagnado, C.A. et al. (1994). “AUUUA Is Not Sufficient to Promote Poly(A) Shortening and Degradation of an mRNA: The Functional Sequence Within AU-Rich Elements May Be UUAUUUA(U/A)(U/A),”Mol. Cell. Biol. 14:7984-7995.
Levy, A.P. et al. (1996). “Post-Transcriptional Regulation of Vascular Endothelial Growth Factor by Hypoxia,”J. Biol. Chem. 271:2746-2753.
Levy, J.R. et al. (1995). “Sequence and Functional Characterization of the Terminal Exon of the Human Insulin Receptor Gene,”Biochim. Biophys. Acta1263:253-257.
Lewis, T. et al. (May 1998). “Mapping of a Minimal AU-Rich Sequence Required for Lipopolusaccharide-Induced Binding of a 55-kDa Protein on Tumor Necrosis Factor-α mRNA,”J. Biol. Chem. 273:13781-13786.
Mitchell, P. et al. (Apr. 2000). “mRNA Stability in Eukaryotes,”Curr. Opin. Genet. Dev. 10:193-198.
Mitchell, P. et al. (Jun. 2001). “mRNA Turnover,”Curr. Opin. Cell. Biol. 13(3):320-325.
Nanbu, R. et al. (1994). “Multiple Instability-Regulating Sites in the 3′ Untranslated Region of the Urokinase-Type Plasminogen Activator mRNA,”Mol. Cell. Biol. 14:4920.
Ross, J. (Sep. 1995). “mRNA Stability in Mammalian Cells,”Microbiol. Rev. 59(3):423- 450.
Sachs,

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