Assay for identifying antibody producing cells

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S069600, C435S070210, C435S071100

Reexamination Certificate

active

07993864

ABSTRACT:
The present invention provides a homogeneous assay for identifying an antibody producing cell producing an antibody which binds to a selected antigen comprising: a) providing a population of antibody producing cells; b) incubating said population of antibody producing cells with a selected antigen and a labeled anti-antibody antibody, wherein said anti-antibody antibody is capable of distinguishing cells producing an antibody which binds to the selected antigen from those cells which do not; and c) identifying an antibody producing cell capable of producing an antibody which binds to the selected antigen.

REFERENCES:
patent: 3865689 (1975-02-01), Goldenberg
patent: 4816397 (1989-03-01), Boss et al.
patent: 5264341 (1993-11-01), Maciak et al.
patent: 5326696 (1994-07-01), Chang
patent: 2007/0243564 (2007-10-01), Lawson et al.
patent: 0 286 405 (1988-10-01), None
patent: WO 92/02551 (1992-02-01), None
patent: WO 94/09117 (1994-04-01), None
patent: WO 99/58977 (1999-11-01), None
patent: WO 02/44216 (2002-06-01), None
patent: 2005/121789 (2005-12-01), None
patent: 1999/57311 (2010-04-01), None
Kipps et al, in Weir et al (Eds.), Handbook of Experimental Immunology, vol. 4, Blackwell Scientific Publications, 1986, pp. 108.1-108.9.
Sternberger, Immunocytochemistry, Prentice-Hall, Inc., 1974, pp. 18-32.
Stephens et al., “Automation for High-Throughput Identification and Picking of GFP Expressing Colonies”, J. Assoc. Laboratory Automation 7(3):41-43 (Jun. 2002).
International Search Report for PCT/GB03/05254 dated Mar. 4, 2004.
International Search Report for PCT/GB2005/002305 dated Nov. 3, 2005.
“Merriam-Webster” online definition for “anti-idiotypic antibody” (pp. 1-2 (Jan. 8, 2009)).
“biology online” definition for “anti-idiotypic antibody” (p. 1 (Jan. 8, 2009)).
“online medical” definition for “anti-idiotypic antibody” (p. 1 (Jan. 8, 2009)).
Babcook, J.S., et al., “A novel strategy for generating monoclonal antibodies from single, isolated lymphocytes producing antibodies of defined specificities,”Proc. Natl. Acad. Sci. USA, 1996, 93, 7843-7848.
Bae, S.W.., et al., “Influence of fluorescent antibody probe specificity on flow cytometric analysis of antibody-producing cells,”J. of Immunol. Methods, 1996, 189(1), 83-88.
Bird, R.E., et al., “Single-chain antigen-binding proteins,”Science, 1988, 242, 423-426.
Holmes, P., et al., “Improved cell line development by a high throughput affinity capture surface display technique to select for high secretors,”J. of Immunol. Methods, 1999, 230, 141-147.
Jerne, N.K., et al., “Plaque formation in agar by single antibody-producing cells,”Science, 1963, 140, p. 405.
Moav, N., et al., “Biologic activities of lymph node cells fractionated on bovine plasma albumin,”J. of Immunol., 1970, 105(6), 1512-152.
Morrison, S.L., et al., “Chimeric human antibody molecules: mouse antigen-binding domains with human constant region domains,”Proc. Natl. Acad. Sci. USA, 1984, 81, 6851-6855.
Orlandi, R., et al., “Cloning immunoglobulin variable domains for expression by the polymerase chain reaction,”Proc. Natl. Acad. Sci. USA, 1989, 86, 3833-3837.
Reading, C.L., “In vitro immunization for the production of antigen-specific lymphocyte hybridomas,”Methods in Enzymology, Langone, J.J., et al. (Eds.),Academic Press Inc., N.Y., 1986, 121, 18-27.
Riechmann, L., et al., “Reshaping human antibodies for therapy,”Nature, 1988, 322, 323-327.
Van Mourik, P., et al., “Improved hybridoma technology: spleen cell separation and soluble growth factors,”Methods in Enzymology, Langone, J.J., et al. (Eds.),Academic Press Inc., N.Y., 1986, 121, 174-182.
Verma, R., et al., “Antibody engineering: comparison of bacterial, yeast, insect and mammalian expression systems,”J. of Immunol. Methods, 1998, 216, 165-181.
Sung, W. B. et al., “Influence of fluorescent antibody probe specificity on flow cytometric analysis of antibody-producing cells”, Journal of Immunological Methods, 1996, 189(1), 83-88.
Ward, E. S. et al., “Binding activities of a repertoire of single immunoglobulin variable domains secreted fromEscherichia coli”, Nature, 1989, vol. 341, 544-547.

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