Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1997-01-10
2000-03-21
Hobbs, Lisa J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
435194, 424 945, C12Q 148, C12N 912, A61K 3851
Patent
active
060401494
ABSTRACT:
The subject invention provides a purified membrane-bound ceramide-activated protein kinase having an apparent molecular weight of about 110 kD as determined by SDS polyacrylamide gel electrophoresis, which protein kinase is capable of specifically phosphorylating the threonine residue in a Thr-Pro- or a Thr-Leu-Pro-containing polypeptide. The subject invention also provides a method of determining whether an agent is capable of specifically inhibiting the phosphorylation activity of the ceramide-activated protein kinase. The subject invention further provides a method of determining whether an agent is capable of specifically stimulating the phosphorylation activity of the ceramide-activated protein kinase. The subject invention further provides a method of treating a subject having an inflammatory disorder. The subject invention further provides a method of treating a human subject infected with HIV so as to reduce the proliferation of HIV in the human subject. The subject invention further provides a method of treating a subject having a disorder associated with poor stem cell growth. The subject invention further provides a method of determining whether an agent is capable of specifically inhibiting the ability of lipopolysaccharide to stimulate the phosphorylation activity of the ceramide-activated protein kinase of the subject invention. Finally, the subject invention provides a method of treating a subject suffering from a lipopolysaccharide-related disorder.
REFERENCES:
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Kolesnick Richard N.
Liu Jun
Zhang Yuhua
Hobbs Lisa J.
Sloan-Kettering Institute for Cancer Research
White John P.
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