Assay for high density lipoprotein cholesterol

Details Assay for high density lipoprotein cholesterol Assay for high density lipoprotein cholesterol

1990-08-06

1991-07-23

Lacey, David L.

Chemistry: analytical and immunological testing

Lipids, triglycerides, cholesterol, or lipoproteins

436 63, 435 11, G01N 504, G01N 3348, G01N 3350

Patent

active

050343327

ABSTRACT:
This invention relates to an assay for high density lipoprotein in blood. More particularly, it relates to the use of boar vesicle seminal plasma protein material to bind to and precipitate HDL cholesterol. In one embodiment, the method involves measuring the cholesterol using standard methods prior to and after precipitation of the HDL. The difference in measurements being an estimate of HDL cholesterol present in the sample.

REFERENCES:
J. Boursnell et al., 11 J. Reprod. Fert., 139-144 (1966).
J. Boursnell et al., 19 J. Reprod. Fert., 157-166 (1969).
D. Schellpfeffer et al., 23 J. Reprod. Fert., 291-198 (1970).
T. Roberts et al., 41 J. Reprod. Fert., 489-492 (1974).
H. Moore et al., 47 J. Reprod. Fert., 39-45 (1976).
Sigma Diagnostics product information sheet for HDL precipitating reagent, date of publication unknown, admitted prior art.
Chapter 6: "Chemistry Studies," pp. 334-337, in Cholesterol, date of publication unknown, admitted prior art.
Sigma Diagnostics pamphlet for Cholesterol Reagent, (1988) by Sigma Chemical Company.

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