Assay for hepatitis B core antibody

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435287, 435810, C12Q 166

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active

042411750

ABSTRACT:
HB.sub.c Ab is detected in biological fluid by contacting HB.sub.c Ag derived from Dane particles and adhered to a surface with the biological fluid and an antibody-enzyme conjugate. The HB.sub.c Ag is adhered to the surface in the presence of a nonionic surfactant.

REFERENCES:
patent: 3551555 (1970-12-01), Schuurs
patent: 3654090 (1972-04-01), Schuurs et al.
patent: 3995019 (1976-11-01), Jerome
patent: 4100267 (1978-07-01), Shaw
patent: 4102996 (1978-07-01), McAleer et al.
patent: 4148869 (1979-04-01), Deaton
patent: 4157280 (1979-06-01), Halbert et al.
Purcell et al, "Radioimmunoassay for the Detection of the Core of the Dane Particle and Antibody to It", Intervirology, vol. 2 (1973/1974) pp. 231-243.
Mathiesen, et al, "Enzyme-Linked Immunosorbent Assay for Detection of Hepatitis A Antigen in Stool and Antibody to Hepatitis A Antigen in Sera: Comparison with Solid-Phase Radioimmunoassay, Immune Electron Microscopy, and Immune Adherence Hemagglutination Assay", J. Clin. Micro., vol. 17, No. 2, (1978), pp. 184-193.

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