Assay for Hepatitis A virus specific antibodies

Details Assay for Hepatitis A virus specific antibodies Assay for Hepatitis A virus specific antibodies

1996-07-05

1998-08-11

Woodward, Michael P.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 4, 435 792, 435 795, C12Q 170

Patent

active

057926050

ABSTRACT:
A new, ultrasensitive, capture EIA based assay--ml-HAV EIA suitable for the detection of vaccine or disease-inducted Hepatitis A virus specific antibodies of IgG class in saliva. The method provides 99% sensitivity and specifically in comparison with serum based assays, and comprises a method of assaying specific immunoglobulin of any or all classes present in a human or animal bodily fluid selected from saliva, tears, semen, urine and cerebro spinal fluid by the steps of:

REFERENCES:
Delem, A., Vaccine 11(4): 479-484, 1993.
Macy et al., "Enhanced ELISA: how to measure less than 10 picograms of a specific protein (immunoglobulin) in less than 8 hours", The FASEB Journal, vol. 2, pp. 3003-3009, Nov. 1988.
Parry, et al., "Sensitive assays for viral antibodies in saliva: an altrnative to tests on serum", The Lancet, Jul. 11, 1987, 72-75.

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