Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-05-31
1998-04-07
Chan, Christina
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 6, 435 29, 435405, 435408, C12N 1501, C12Q 168, G01N 33483
Patent
active
057363455
ABSTRACT:
A novel bronchial or bronchiolar epithelial cell from normal neonatal mammalian lung has been isolated, established and maintained for multiple passages in the absence of serum, without undergoing crisis or senescence. By careful manipulation of the nutritional/hormonal microenvironment we have been able to select, from a heterogeneous population, a single epithelial cell type which can maintain highly differentiated features in vitro. This cell type has characteristics of bronchiolar epithelial cells. A clonal line, RL-65, has been selected and observed for more than 3 years in continuous culture. It has been characterized by ultrastructural, morphological and biochemical criteria.
REFERENCES:
patent: 4885238 (1989-12-01), Reddel et al.
patent: 4889808 (1989-12-01), Rappaport
patent: 5089397 (1992-02-01), Kushner et al.
patent: 5356806 (1994-10-01), Harris et al.
patent: 5364785 (1994-11-01), Mather et al.
Baserga, R.; In: Cell growth and division. Baserga, R. editor. Chapter 1. IRL Press, Oxford, UK, 1989.
Greenwood, D, et al; In: Cell growth and division. Baserga, R. editor. Chapter 1. IRL Press, Oxford, UK, 1989.
Patton, et al. Experimental Lung Research 11:277-294, 1986.
Klann et al., "Effects of Retinoic Acid on Cell Proliferation and Cell Differentiation in a Rat Tracheal Epithelial Cell Line" Cell Tissue Kinet 15:473-482 (1982).
Cascieri et al., "Inability of a Mouse Cell Line Transformed to Produce Biologically Active Recombinant Human Insulin-Like Growth Factor I (IGF-I) to Respond to Exogenously Added IFG-I", Endocrinology 122(4):1314-1320 (1988).
Celis et al., "Computerized, Comprehensive Databases of Cellular and Secreted Proteins From Normal Human Embryonic Lung MRC-5 Fibroblasts: Identification of Transformation and/or Proliferation Sensitive Proteins", Electrophoresis 10:76-115 (1989).
Chopra et al., "Propagation of Differentiating Normal Human Tracheobronchial Epithelial Cells in Serum-Free Medium" Journal of Cellular Physiology 130:173-181 (1987).
Evans et al., "Lung Cell Kinetics" Lung Cell Biology pp. 1-89 (1989).
Frehney, "Culture of Specific Cell Types" Culture of Animal Cells A Manual of Basic Technique pp. 267-268 (1987).
Honegger et al., "Insulin-like Growth Factors I and II in Fetal and Adult Bovine Serum" Journal of Biological Chemistry 261(2):569-574 (1986).
Jones et al., "Benzo(a)pyrene Hydoxylase Activity in Enriched Populations of Clara Cells and Alveolar Type II Cells From Control and .beta.-Napthoflavone-pretreated Rats" Cancer Research 42:4658-4663 (1982).
Lechner et al., "Clonal Growth of Normal Adult Human Bronchial Epithelial Cells at Clonal Density" Journal of Tissue Culture Methods 9 (2):43-48 (1985).
Lechner et al., "A Serum-free Method for Culturing Normal Human Bronchial Epithelial Cells at Clonal Density" Journal of Tissue Culture Methods 9(2) 43-48 (1985).
Lee et al., "Growth and Differentiation of Hamster Tracheal Epithelial Cells in Culture" Experimental Lung Research 6:27-45 (1984).
Loo et al., "Extended Culture of Mouse Embryo Cells Without Senescence: Inhibition of Serum" Science 236:200-202 (Apr. 10, 1987).
Mass et al., "The Effect of 12-O-Tetradecanoylphorbol-13-acetate and other Tumor Promoters on the Colony of Formation of Rat Tracheal Epithelial Cells in Culture" Carcinogenesis 5 (12):1597-1601 (1984).
Massaro, "Nonciliated Bronchiolar Epitelial (Clara) Cells " Lung Cell Biology pp. 81-114 (1989).
Massaro et al., "Development of Bronchiolar Epithelium in Rats" American Journal of Physiology 250 (5):783-788 (1986).
Mather et al., "Hormones and Growth Factors in Cell Cultures: Problems and Perspectives" Cell, Tissue and Organ Cultures in Neurobiology, Federoff & Hertz pp. 621-630 (1977).
Netter, "Ultrastructure of Tracheal Bronchial and Bronchiolar Epithelium" The CIBA Collection of Medical Illustrations 7:26 (1979).
Piltch et al., "A Cloned Rat Thymic Epithelial Cell Line Established From Serum-Free Selective Culture" In Vitro Cellular & Developmental Biology 24 (4) :289-293 (1988).
Plopper et al., "Ultrastrucure of the Nonciliated Bronchiolar Epithelial (Clara) cell of Mammalian Lung: I. A Comparison of Rabbit, Guinea Pig, Rat, Hamster, and Mouse" Experimental Lung Research 1:139-154 (1980).
Roberts et al., "A Novel Epithelial Cell From Neonatal Rat Lung: Isolation and Differentiated Phenotype" Properties of Lung Epithelial Cells pp. L415-L425 (1990).
Schumann et al., "Isolation, Characterization, and Long-term Culture of Fetal Bovine Tracheal Epithelial Cells" In Vitro Cellular & Devopmental Biology 24 (3):211-216 (1988).
Scopes, "Analysis for Purity; Crystallization" Protein Purification Principles and Practice, Springer Advanced Texts in Chemistry p. 248 (1983).
Siegfried, "Detection of Human Lung Epithelial Cell Growth Factors Produced by a Lung Carcinoma Cell Line: Use in Culture of Primary Solid Lung Tumors.sup.1 " Cancer Research 47:2903-2910 (Jun. 1, 1987).
Siegfried et al., "Cytotoxicity of chemical Carcinogens Towards Human Bronchial Epithelial Cells Evaluated in a Clonal Assay" Carcinogenesis 5(10):1317-1322 (1984).
Singh et al., "A Quantitative Assay for a Clara Cell-Specific Protein and Its Application in the Study of Development of Pulmonary Airways in the Rat" Pediatric Research 20 (8):802-805 (1986).
Thomassen et al., "Clonal Proliferation of Rat Tracheal Epithelial Cells in Serum-free Medium and Thier Responses to Hormones, Growth Factors and Carcinogens" Carcinogenesis 7(12):2033-2039 (1986).
Tsao et al., "Clonal growth of normal human epidermal keratinocytes in a defined medium" J. Cellular Physiology 110:219-229 (1982).
Willey et al., "Bombesin and the C-Terminal Tetradecapeptide of Gastrin-releasing Peptide are Growth Factors for Normal Human Bronchial Epithelial Cells" Exp. Cell Res. 153:245-248 (1984).
Wu et al., "Continuous Multiplication of Rabbit Tracheal Epithelial Cells in a Defined Hormone-Supplemented Medium" In Vitro 18(9):800-812 (1982).
Wu et al., "Effects of Retinoids on Human Bronchial Epithelial Cells: Differential Regulation of Hyaluronate Synthesis and Keratin Protein Synthesis" J. Cellular Physiology 127:73-82 (1986).
Wu et al., "Expression of Tracheal Differentiated Functions in Serum-free Hormone Supplemented Medium" J. Cellular Physiology 125:167-181 (1985).
Mather Jennie P.
Roberts Penelope E.
Chan Christina
Conley Deidre L.
Genentech Inc.
Lee Wendy M.
VanderVegt F. Pierce
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