Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1993-12-28
1996-01-16
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
4242781, 435 78, 435 794, 435975, 436501, 436513, 436518, 514 11, 514885, G01N 33543, G01N 33566
Patent
active
054847079
ABSTRACT:
Methods of monitoring and detecting the early onset of organ injury incident rejection of a organ rejection in an animal are disclosed. The described methods are capable of distinguishing organ rejection injury from other organ tissue damage in the animal. Free secretory component levels in an animal biological fluid (e.g., bile, urine, blood, amniotic fluid) may be used to identify organ rejection in an animal. Multiple and single organ transplant patients may be monitored and diagnosed according to the claimed methods. Biological fluids, such as blood, (serum) or urine, are analyzed immunologically using a particularly adapted ELISA which are then compared to an FSC control concentration to identify elevated FSC values. Animals with test FSC above FSC control concentrations are diagnosed as having an ongoing organ rejection episode. The detection of congenital renal dysfunction in utero is also provided according to the present invention through the measurement of FSC in the amniotic fluid. The described methods are specific for indicating organ rejection tissue injury, and distinguish kidney rejection tissue injury, in particular, from other causes of kidney injury, such as cyclosporin toxicity, urinary tract infection, and urinary obstruction and toxicity (incident to immunosuppressive therapy with cyclosporin). A kit for use in the identification of an organ rejection episode in a patient through measurement of FSC in a biological sample is also provided.
REFERENCES:
J. Floege et al, Clin. Physiol. Biochem., 7, 165-175, 1989.
C. Vincent et al, Jour. Immunol. Meth., 106, 153-160, 1988.
M. G. Scott, Trends In Biotechnology, 3, 170-175, 1985.
A. Voller, In E. T. Maggio (Ed.) Enzyme Immunoassay, Chapter 9, CRC Press, Inc., Boca Raton, Fla., 1980, pp. 181-196.
Goldblum Randall M.
Rajaraman Srinivasan
Saunders David
The Board of Regents The University of Texas System
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