Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1991-08-26
1993-07-20
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 18, 435 23, 435218, 435184, C12Q 137, C12Q 966, G01N 3353, C12N 999
Patent
active
052292673
ABSTRACT:
N-substituted azetidinones are a class of inhibitors of human leukocytes elastase which are known to be useful in the treatment of a wide variety of antiinflammatory and antidegenerative diseases. In inhibiting elastase, the therapeutic agents are shown to form a characteristic stable complex with the enzyme. In the assay disclosed herein, the inhibitor-enzyme complex is advantageously hydrolyzed and specific product(s) of the hydrolysis are measured. The assays are useful in a clinical setting, for determining appropriate dosage and assessing the effectiveness of treatment.
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Hanlon William A.
Humes John L.
Knight Wilson B.
Mumford Richard A.
Green Lora M.
Kepplinger Esther L.
Merck & Co. , Inc.
Panzer Curtis C.
Speer Raymond M.
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