Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1996-05-06
1998-02-17
Kight, John
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
435 4, 435 772, 435 74, 424 941, 424529, C12Q 148
Patent
active
057190356
ABSTRACT:
The present invention is a method for assaying enzyme activity in a red blood sample. The method comprises these steps: (a) placing the following in a sample well: (1) a red blood sample containing an enzyme, (2) a substrate or substrates for the enzyme, (3) water, and (4) a buffer; (b) incubating the contents of the sample well for sufficient time and at sufficient temperature to allow for the formation of a fluorescent enzyme product should the enzyme be present in said red blood sample; (c) precipitating the hemoglobin; and (d) measuring the fluorescence of any fluorescent enzyme product formed in the sample well, directly from that sample well. The method of the invention may be used for assaying the activity of an enzyme, such as galactose-1-phosphate uridyl transferase (GALT) or biotinidase, in a red blood sample.
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Akhaury Ranjan
Rosenthal Murray A.
Simkins Ronald A.
Isolab, Inc.
Jones Dameron
Kight John
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