Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1994-06-01
1996-07-23
Weimar, Elizabeth C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
435 4, 435 14, 435 25, 435 26, 436 68, 436 95, 436164, 436172, 530380, 530385, 424 941, 424529, 424533, C12Q 148, G01N 3300, A61K 3514
Patent
active
055388574
ABSTRACT:
The present invention is a method for assaying enzyme activity in a red blood sample. The method comprises these steps: (a) placing the following in a sample well: (1) a red blood sample containing an enzyme, (2) a substrate or substrates for the enzyme, (3) water, and (4) a buffer; (b) incubating the contents of the sample well for sufficient time and at sufficient temperature to allow for the formation of a fluorescent enzyme product should the enzyme be present in the red blood sample; (c) precipitating the hemoglobin; and (d) measuring the fluorescence of any fluorescent enzyme product formed in the sample well, directly from that sample well. The method of the invention may be used for assaying the activity of an enzyme, such as galactose-1-phosphate uridyl transferase (GALT) or biotinidase, in a red blood sample.
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Akhaury Ranjan
Rosenthal Murray A.
Simkins Ronald A.
Isolab, Inc.
Mohamed Abdel A.
Weimar Elizabeth C.
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