Assay for enzyme activity

Details Assay for enzyme activity Assay for enzyme activity

1994-06-20

1996-04-30

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 19, 435 18, 435962, 435975, 436 60, 436 63, 436 71, 436501, 436 37, C12Q 100, C12Q 144, G01N 3353, G01N 3100

Patent

active

055124291

ABSTRACT:
A method of assay for an enzyme catalysing the release of long chain fatty acids or, conversely, for an ester substrate for such enzymes is applicable to clinical samples and comprises removing substantially all the albumin from the clinical sample, incubating the sample after albumin removal with, for enzyme assay, an ester substrate or, for substrate assay, the enzyme, under conditions effective to release a fatty acid, causing the fatty acid to bind to a fatty acid binding protein and assaying the binding of the fatty acid to the binding protein.

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