Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-12-05
1997-07-29
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 435 912, 536 237, 536 2432, 536 2433, C07H 2102, C07H 2104, C12P 1934, C12Q 168
Patent
active
056521022
ABSTRACT:
Primers specific for enterohemorrhagic Escherichia coli (EHEC) 0157:H7 bacteria have been designed which are useful for detecting the bacteria by polymerase chain reaction methods. The primers were derived from DNA sequences contained within a 60-MDa plasmid which is present in most EHEC. The primers may also be used in combination with primers derived from other sequences of significance, the conserved sequences of Shiga-like toxins I and II and the eaeA gene, in a single simultaneous amplification reaction to specifically identify EHEC serotype 0157.
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Fratamico Pina M.
Sackitey Solomon K.
Wiedmann Martin
Fado John
Graeter Janelle S.
Silverstein M. Howard
The United States of America as represented by the Secretary of
Tran Paul B.
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