Assay for direct binding of peptides that are T-cell epitopes to

Details Assay for direct binding of peptides that are T-cell epitopes to Assay for direct binding of peptides that are T-cell epitopes to

1990-12-10

1994-10-18

Saunders, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 721, 435 75, 435 78, 435 29, 436503, 436506, G01N 33564, G01N 33567

Patent

active

053567790

ABSTRACT:
As assay for the measurement of direct binding of a peptide that is a T-cell epitope to gene products of the major histocompatibility complex (MHC), classes I and II, on the surface of intact living antigen-presenting cells, is provided. The assay comprises incubating the labelled peptide with the cells, and monitoring the extent of binding by the addition of a probe that reacts with the ligand used to label the peptide. The assay is suitable for autoimmune diseases and other immunological disorders. Peptides having a sequence corresponding to a stretch of the sequence of the antigen relevant to an immunological disorder, or modifications thereof, which bind to gene products of MHC, but do not stimulate T-cells, are also useful for the treatment of said disorders.

REFERENCES:
patent: 5200320 (1993-04-01), Sette et al.
I. M. Roitt, Essential Immunology-5th Ed., Blackwell Scientific Publications, Oxford, UK, 1984, pp. 65-66.
J. B. Rothbard et al, Internat. Immunol., l1, 479-486, 1989.
E. Mozes et al, EMBO Journal, 8, 4049-4052 (1989).
D. E. Smilek et al, Immunol. Rev., 118, 37-71 (1990).
R. Busch et al, Internat. Immunol., 2, 444-451 (1990).
Chemical Abstracts, vol. 112, No. 19, May 1990, p. 537, abstract No. 176542y, Columbis, Ohio, Us; E. Mozes et al. "Direct binding of a myasthenia gravis related epitope to MHC class II molecules on living murine antigen-presenting cells", & EMBO J. 1989, 8(13), 4049-52.
Nature, vol. 339, Jun. 1989, pp. 392-394, London, GB; R. Ceppellini et al. "Binding of labelled influenza matrix peptide to HLA DR in living Bl Lymphoid cells".

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