Assay for dioxins

Details Assay for dioxins Assay for dioxins

1989-01-25

1992-07-07

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 793, 435975, 436501, 436503, 436504, 436545, 436804, 436808, 436822, 549339, G01N 3353

Patent

active

051282445

ABSTRACT:
A screening assay for recognizing the presence of dioxins (and other related toxins) in a sample is disclosed. In one aspect of the invention, Ah receptor from mice and a radioactively labelled halogenated dioxin are used in a competitive binding assay to test for the presence of toxins. The label is .sup.125 I substituted directly on the main dioxin structure. The relative binding of the toxin in the samples (in competition with labelled dioxin) for Ah receptor can be compared against standard curves. A kit is provided for running such an assay and a preferred .sup.125 I ligand is provided.

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F. Ebetino, Design of a Probe for the TCDD Receptor, University of Rochester, Part II of Doctoral Thesis, pp. 71-124 (pp. i-vii) (1984).
A. Poland et al., 251 J. Biol. Chem. 4936-4946 (1976).
A. Poland et al., 261 J. Biol. Chem. 6352-6365 (May 15, 1986).
J. Denny et al., 81 P.N.A.S. USA 5286-5290 (1984).
J. Cadogan et al., J. Chem. Soc. (Perkins 1) 541-42 (1973).

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