Assay for determining sensitivity to activated protein C

Details Assay for determining sensitivity to activated protein C Assay for determining sensitivity to activated protein C

1995-05-01

1997-07-01

Tsang, Cecilia J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving blood clotting factor

435810, 436 69, 436808, 514834, 530381, 530383, 530384, 424529, C12Q 156, G01N 3386, C07K 100

Patent

active

056437390

ABSTRACT:
An improved assay for determining sensitivity to activated protein C in test samples has been developed to ensure rapid and accurate evaluations. This assay is based on measuring the conversion, by activated factor VIII within a test sample, of added factor X to an activated form. The activated protein C sensitivity of the test sample is determined by the relative inhibition of factor X conversion as compared to a control. A test sample that has decreased sensitivity to activated protein C will show relatively low inhibition, and vice versa.

REFERENCES:
patent: 4672030 (1987-06-01), Witt
Wagenvoord et al., Haemostasis, vol. 19, pp. 196-204, 1989.
Esmon, "Protein S and Protein C: Biochemistry, Physiology, and Clinical Manifestation of Deficiencies," TCM, vol.2, No. 6 (1992).
Dahlback et al., "Familial Thrombophilia Due to a Previously Unrecognized Mechanism Characterized by Poor Anticoagulant Response to Activated Protein C: Prediction of a Cofactor to Activated Protein C", Proc. Natl. Acad. Sci., vol. 90, pp. 1004-1008 (1993).
Bokarewa et al., "Heterogeneity of the APC-Resistance Phenomenon", Thrombosis Research, vol. 75, No. 4, pp. 395-400, (1994).
Shen et al., "Factor V and Protein S As Synergistic Cofactors To Activated Protein C In Degradation of Factor VIIIa", The Journal of Biological Chemistry, vol. 269, No. 29, pp. 18735-18738, (1994).
Varadi et al., "A Chromogenic Assay For Activated Protein C Resistance", British Journal of Haematology, vol. 90:884-891, (1995).

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