Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1998-03-17
2000-10-24
Minnifield, Nita
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 732, 435 792, 435 5, 436516, 436528, 436531, 436532, 422 55, 422 56, 422 57, 4242361, 4242391, 4242471, 204456, 204462, G01N 3353, G01N 33561, G01N 33544, A61K 3902, C12Q 170
Patent
active
061365513
ABSTRACT:
An assay for the identification of neutralizing botulinum antibodies in sera is provided which includes the steps of separating non-sodium dodecyl sulfate, non-trypsinized complex botulinum toxin in an arylamide gel by electrophoresis, the separation occurring on a basis of botulinum toxin protein size and charge. Thereafter, the separated protein is electrophoretically transferred onto a solid support, the transferred separated protein being bound to the solid support at spaced apart sites. Remaining sites on the solid support not occupied by bound protein are blocked and the solid support and bound protein are contacted with a sera sample containing an antibody directed against botulinum toxin. The contacted solid support is then exposed to a second antibody capable of reacting to produce an insoluble colored substrate of intensity relative to a quantity of antibodies present. Finally, the second antibody is reacted to produce the insoluble colored substrate which is visually identified.
REFERENCES:
patent: 4452901 (1984-06-01), Gordon et al.
De Maio, A. protein blotting and immunoblotting using nitrocellulose membranes. in:Dunbar,B.S, Protein blotting: a practical approach (New York, Oxford University press 1994, pp. 11-14.
Siegel S, Lynn, Human immune response to botulinum pentavalent toxoid determined by a neutralization test and by an enzyme linked immunosorbent assay. Jou.Clinical microbiology, 26, (11) 2351-2356, 1988.
Aoki Kei Roger
Spanoyannis Athena F.
Allergan Inc.
Baskar Padma
Hackler Walter A.
Minnifield Nita
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