Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Reexamination Certificate
2006-09-19
2006-09-19
Gitomer, Ralph (Department: 1655)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
Reexamination Certificate
active
07108997
ABSTRACT:
The invention is an assay for detecting inhibitors of the enzyme myeloperoxdase The assay method comprises reacting myeloperoxdase with hydrogen peroxide and a chloride source to generate hypochlorous acid in the presence of a potential inhibitor of said myeloperoxidase; reacting any formed hypochlorous acid with an amine to form the corresponding chloramino; optionally removing any unreacted hydrogen peroxide; reacting any for-med chloramine with a detector compound in the presence of iodide to form oxidized detector compound; determining the amount of formed oxidized detector compound by measuring the absorbents or fluorescence at a suitable wavelength; and identifying as inhibitors of myeloperoxidase those compounds which cause the amount of formed oxidized detector compound to be decreased The assay is useful in diagnostic tests for myeloperoxidase activity.
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AstraZeneca AB
Gitomer Ralph
Kondrad Karen H.
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