Assay for detecting inhibitors of aminoacyl-tRNA synthetases

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Enzyme inactivation by chemical treatment

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435 21, 435 692, C12N 999

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059856300

ABSTRACT:
An assay for detecting inhibitors of amino acyl-tRNA synthetases by incubating a divalent metal cation, ATP, tRNA, a non-cognate amino acid, inorganic pyrophosphatase and aminoacyl-tRNA synthetase, which aminoacyl-tRNA synthetase is in at least a partially pure form, both with and without a potential inhibitor, converting the resulting pyrophosphate to phosphate, detecting phosphate production, and comparing the phosphate production results obtained.

REFERENCES:
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Freist, Wolfgang and Sternbach, Hans, "Isoleucyl-tRNA Synthethase from Bakers' Yeast: Variable Discrimination between tRNA and tRNA and Different Pathways of Cognate and Noncognate Aminoacylation under Standard Conditions, in the Presence of Pyrophosphatase, Elongation Factor Tu-GTP Complex, and Spermine", Biochemistry, 1984, 23, pp. 5742-5752.

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