Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Enzyme inactivation by chemical treatment
Patent
1997-08-05
1999-11-16
Gitomer, Ralph
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Enzyme inactivation by chemical treatment
435 21, 435 692, C12N 999
Patent
active
059856300
ABSTRACT:
An assay for detecting inhibitors of amino acyl-tRNA synthetases by incubating a divalent metal cation, ATP, tRNA, a non-cognate amino acid, inorganic pyrophosphatase and aminoacyl-tRNA synthetase, which aminoacyl-tRNA synthetase is in at least a partially pure form, both with and without a potential inhibitor, converting the resulting pyrophosphate to phosphate, detecting phosphate production, and comparing the phosphate production results obtained.
REFERENCES:
patent: 4879223 (1989-11-01), Miyazawa
patent: 4886749 (1989-12-01), Dombou
Malathi V, An Inhibitor which Interferes with the . . . Biochim Et Biophys Acta 517 (1978) 228-235.
Fersht, Alan R., "Editing Mechanisms in Protein Synthesis. Rejection of Valine by the Isoleucyl-tRNA Synthethase", Biochemistry, vol. 16, No. 5, (Mar. 8, 1977), pp. 1025-1030.
Fersht, Alan R., et al., "Enzyme Hyperspecificity, Rejection of Threonine by the Valyl-tRNA Synthethase by Misacylation and Hydrolytic Editing", Biochemistry, vol. 15, No. 15 (Jul. 27, 1976), pp. 3342-3346.
Howard, Joy L. et al., "Acetyl-CoA carboxylase: a rapid novel assay procedure used in conjunction with the preparation of enzyme from maize leaves", FEBS, vol. 261, No. 2 (1990), pp. 261-264.
Baldwin, Anne Norris, et al., "Transfer Ribonucleic Acid-induced Hydrolysis of Valyladenylate Bound to Isoleucyl Ribonucleic Acid Synthase", Journal of Biological Chemistry, vol. 241, No. 4 (1966), pp. 839-845.
Lanzetta, Peter A., Alvarez, Lawrence J., Reinach, Peter S., and Candia, Oscar A., "An Improved Assay for Nanomole Amounts of Inorganic Phosphate", Analytical Biochemistry, vol. 100, No. 1, Nov. 15, 1979, pp. 95-97.
Yanagisawa, Tatsuo, Lee, Judy T., Wu, Henry C., and Kawakami, Makoto, Relationship of Protein Structure of Isoleucyl-tRNA Synthetase with Pseudomonic Acid Resistance of Escherichia coli, Journal of Biological Chemistry, vol. 269, No. 39, pp. 24304-24309.
Freist, Wolfgang and Sternbach, Hans, "Isoleucyl-tRNA Synthethase from Bakers' Yeast: Variable Discrimination between tRNA and tRNA and Different Pathways of Cognate and Noncognate Aminoacylation under Standard Conditions, in the Presence of Pyrophosphatase, Elongation Factor Tu-GTP Complex, and Spermine", Biochemistry, 1984, 23, pp. 5742-5752.
Gitomer Ralph
Thomson Marian T.
Zeneca Limited
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