Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-11-27
2000-01-11
Campbell, Eggerton A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 71, C12Q 168, G01N 3353
Patent
active
060134380
ABSTRACT:
The invention relates to the detection of apoptotic cells using a novel assay that employs ligation of DNA fragments in situ to selectively label apoptotic cells. The assay may be used in combination with known in situ methodologies to simultaneously detect apoptotic cells and specific biomolecules present in the apoptotic cell.
REFERENCES:
patent: 5330972 (1994-07-01), Cope
patent: 5484710 (1996-01-01), Reed et al.
patent: 5500432 (1996-03-01), Nicolaou et al.
patent: 5512435 (1996-04-01), Renschler et al.
patent: 5527682 (1996-06-01), Owens et al.
patent: 5624808 (1997-04-01), Thompson et al.
J. Pathol. 170(1):1-8 (Ansari et al) 1993.
Arends, et al., American Journal of Pathology 136(3), 593-608, 1990. Apoptosis: The role of the Endonuclease.
Columbano, A., Journal of Cellular Biochemistry 58, 181-190, 1995. Cell Death: Current Difficulties in Discriminating Apoptosis from Necrosis in the Context of Pathological Processes In Vivo.
Didenko, et al., Journal of Clinical Investigation 97(7), 1723-1731, 1996. Expression of p21.sup.WAFI/CIPI/SDII and p53 in Apoptotic Cells in the Adrenal Cortex and Induction by Ischemia/Reperfusion Injury.
Didenko, et al., Journal of Histochemistry and Cytochemistry 44(6), 657-660, 1996.A Quantitative Luminescence Assay for Nonradioactive Nucleic Acid Probes.
Gavrieli, et al., Journal of Cell Biology 119(3), 493-502, 1992. Identifiaction of Programmed Cell Death In Situ via Specific Labelling of Nuclear DNA Fragmentation.
Grasil-Kraupp, et al., Hepatology 21, 1465-1468, 1995, In situ Detection of Fragmented DNA (TUNEL Assay) Fails to Discriminate Among Apoptosis, Necrosis, and Autolytic Cell death: A Cautionary Note.
Lutter, Nucleic Acids Research 6(1) 41-56, 1979. Precise location of DNase I cutting sites in the nucleosome core determined by high resolution gel electrophoresis.
Sasano, H., Endocrine Pathology 6(2), 87-89, 1995. In Situ End Labeling and Its Applications to the Study of Endocrine Disease: How Can We Study Programmed Cell Death in Surgical Pathology Materials.
Sollner-Webb, et al., Cell 14, 611-627, 1978. DNase I, DNase II and Staphylococcal Nuclease Cut at Different, Yet Symmetrically Located, Sites in the Nucleosome Core.
Van Lookeren Campagne, et al., European Journal of Neuroscience 7, 1627-1640, 1995. NMDA and Kainate Induce Internucleosomal DNA Cleavage Associated with Both Apoptotic and Necrotic Cell Death in the Neonatal Rat Brain.
Yang, et al., Experimental Cell Research 221, 126-131, 1995. Increased expressin of p21.sup.SdII in Adrenocortical Cells When They are Placed in Culture.
Yasuda, M., et al., Archives of Histology and Cytology 58(2), 185-190, 1995. Apoptotic Cells in the Human Endometrium and Placental Villi: Pitfalls in Applying the TUNEL Method.
Didenko Vladimir
Hornsby Peter
Baylor College of Medicine
Campbell Eggerton A.
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