Assay for chlamydia trachomatis by amplification and detection o

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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536 231, C12Q 168, C07H 2102

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058374698

ABSTRACT:
A region of the Chlamydia trachomatis ltuB gene has been identified which is useful for performing amplification assays to determine specifically whether C. trachomatis is present in the sample being tested. Oligonucleotides useful for performing thermophilic Strand Displacement Assay (tSDA) reactions on this gene are disclosed. The disclosed oligonucleotides can be used in an assay which is specific for all strains of C. trachomatis and which does not show crossreactivity with the genomes of other microorganisms or with human DNA.

REFERENCES:
Fahr et al J. Bacteriol 177(15):4252-4260, 1995.

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