Assay for Chlamydia trachomatis by amplification and detection o

Details Assay for Chlamydia trachomatis by amplification and detection o Assay for Chlamydia trachomatis by amplification and detection o

1997-11-04

2000-08-01

Carlson, Karen Cochrane

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 536 2433, C12Q 168, C12P 1934, C07H 2104

Patent

active

060965010

ABSTRACT:
A region of the Chiamydia trachomatis cryptic plasmid has been identified which is useful for performing amplification assays to determine specifically whether C. trachomiltis is present in the sample being tested. Oligonucleotides useful for performing thermal Strand Displacement Assay (tSDA) reactions on this gene are disclosed. The disclosed oligonucleotides can be used in an assay which is specific for all strains of C. trachomatis and which does not show crossreactivity with the genomes of other microorganisms or with human DNA.

REFERENCES:
New England BioLabs Catalog 96/97, pp. 111 and 121, 1996.
Spears, Patricia A. et al., Analytical Biochemsitry; 247, pp. 130-137 (1997).
Walker, G.T. et al., Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system; PNAS, 89, pp. 392-396 (1992).
Walker, G.T. et al., Strand Displacement amplification--an isothermal, in vitro DNA amplification technique; Nucl. Acids Res., 20, pp. 1691-1696 (1992).

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