Assay for cardiac troponin I

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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5303891, 5303881, 53038815, 5303871, 530413, C07K 1600

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active

057566828

ABSTRACT:
A method for detection of cardiac troponin I is described. The method is a sandwich assay employing an antibody specific for cardiac troponin I as one binding partner and troponin C as the other binding partner for cardiac troponin I.

REFERENCES:
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patent: 5290678 (1994-03-01), Jackowski
Larue, Catherine et al. (1993) "Cardiac-Specific Immunoenzymometric Assay of Troponin I in the Early Phase of Acute Myocardial Infarction" Clin. Chem 39(6):972-979.
Armour, K. et al. (1993) "Cloning and Expression in Escherichia Coli of the cDNA encoding Human Cardiac Troponin I" GENE 131:287-292.
Bodor et al. (1992) "Development of Monoclonal Antibodies for an Assay of Cardiac Troponin-I and Preliminary Results in Suspected Cases of Myocardial Infarction" Clin. Chem. 38(11);2203-2214.
Cummins, B, and P Cummins (1987) "Cardiac Specific Troponin-I Release in Canine Experimental Myocardial Infraction: Development of a Sensitive Enzyme-Linked Immunoassay" J. Mol. Cell Cardiol. 19(10):999-1010.
Cummins, B. and P. Cummins (1987) "Immunoassay of the Cardiac-Specific Isoform of Tropinin-I in the Diagnosis of Heart Muscle Damage" Biochemical Society Transactions 15(6):1060-1061.
Cummins et al. (1987) "Cardiac-Specific Tropinin-I Radioimmunoassay in the Diagnosis of Acute Myocardial Infarction" Amer. Heart J. 113(6):1333-1344.
Larue, C. et al. (1992) "New Monoclonal Antibodies as Probes for Human Cardiac Troponin I: Epitopic Analysis with Synthetic Peptides" Molecular Immunology 29(2):271-278.
Potter (1982) "Preparation of Troponin and Its Subnits" Methods in Enzymology 85:241-263.
Syska et al. (1994) "A New Method of Preparation of Troponin I (Inhibitory Protein) Using Affinity Chromatography . Evidence for Three Different Forms of Troponin in Striated Muscle" FEBS Letters 40(2):253-257.
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