Assay for bone alkaline phosphatase

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 794, 435 21, 435975, 436518, 436548, 436808, 53038826, 5303911, 5303913, G01N 33573

Patent

active

055254730

ABSTRACT:
Monoclonal antibodies highly specific for human bone alkaline phosphatase, especially in the presence of human liver alkaline phosphatase, and their use in assays for human bone alkaline phosphatase are disclosed. A kit using the antibodies in an assay for human bone alkaline phosphatase is also disclosed.

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Masuhara et al., "Purification Of Bone Alkaline Phosphatase From Human Osteosarcoma" Bone and Mineral. 3:159-170 (1987)
Bailyes et al., "The Preparation Of Monoclonal Antibodies To Human Bone and Liver Alkaline Phosphatase And Their Use In Immunoaffinity Purification And In Studying These Enzyme When Present In Serum" Biochemistry Journal vol. 244:725-733 (1987).
Nerurkar et al., "Rapid Detection Of Herpes Simplex Virus In Clinical Specimens By Use Of A Capture Biotin-Streptavidin Enzyme-Linked Immunosorbet Assay" Journal of Clinical Microbiology. vol. 20, No.1:109-114 (1984).
Seabrook et al., "The Distinction Of Bone And Liver Isoenzyme Of Alkaline Phosphatase In Serum Using A Monoclonal Antibody" Clinica Chimica Acta. 172:261-266 (1988).
Lawson et al.,"Isolation And Preliminary Characterization Of A Monoclonal Antibody That Interacts Perferentially With The Liever Isoenzyme Of Human Alkaline" Clin. Chem. vol. 31 No. 3:381-385 (1985).

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