Assay for Beta-lactam antibiotics

Details Assay for Beta-lactam antibiotics Assay for Beta-lactam antibiotics

1985-05-16

1988-08-09

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435188, 435810, 435820, 436501, 436503, 436518, 436548, 436808, 436815, 436822, 530391, 530807, G01N 33535, G01N 33543, G01N 33566, G01N 33577

Patent

active

047627824

ABSTRACT:
An improved assay for measuring the quantity of Beta-lactam antibiotics, particularly valuable for measuring penicillin in milk. The assay includes the covalent-attachment of a penicillin to a penicillin-binding-protein bound to a matrix, the highly selective attachment of an antibody to such a conjugate, and the detection and measurement of the quantity of resulting antibody-complex material as a direct measure of the amount of penicillin in the milk being tested.

REFERENCES:
patent: 4596768 (1986-06-01), Singh et al.
G. Kleppe et al, Journ. Biol. Chem., 254, 4856-4862, 1979.

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