Assay for antigens by binding immune complexes to solid supports

Details Assay for antigens by binding immune complexes to solid supports Assay for antigens by binding immune complexes to solid supports

1987-12-21

1990-09-25

Spiegel, Jack

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 29, 435871, 436510, 436511, 436518, 436531, 436538, 436539, 436540, 436541, 436543, 436824, 436828, G01N 3353, G01N 33536, G01N 33537, C12Q 102

Patent

active

049593030

ABSTRACT:
Methods are disclosed for detecting an antigen in a biological sample. The methods involve providing in combination a solid support, which is substantially free of specific binding proteins, and a medium comprising an antigen from the sample and an antibody for the antigen. The combination is incubated under conditions sufficient for the antibody when bound to the antigen to bind to the support. The presence or amount of antibody on the support or in the medium is determined and is related to the presence of antigen in the sample. The methods have particular application to the detection of gram-negative bacteria.

REFERENCES:
patent: 4254082 (1981-03-01), Schick et al.
patent: 4255412 (1981-03-01), Albert
patent: 4283383 (1981-08-01), Masson et al.
patent: 4427782 (1984-01-01), Caldwell et al.
patent: 4450231 (1984-05-01), Ozkan
patent: 4503143 (1985-03-01), Gerber et al.
patent: 4666863 (1987-05-01), Edwards et al.
patent: 4757024 (1988-07-01), Roper

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