Assay for anti-metastatic agents

Drug – bio-affecting and body treating compositions – In vivo diagnosis or in vivo testing – Diagnostic or test agent produces in vivo fluorescence

Reexamination Certificate

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Details

C435S004000, C435S006120, C435S008000

Reexamination Certificate

active

07829066

ABSTRACT:
This invention relates, e.g., to a method for identifying an agent that inhibits a metastatic cell (e.g. that inhibits cancer metastasis), comprising measuring the amount of seeding of a tumor by a detectably labeled, metastatic cell, in a subject, in the presence and absence of a putative agent, wherein the amount of seeding by the metastatic cell is proportional to the metastatic potential of the cell, and wherein a significant amount of inhibition of the seeding by the putative agent indicates that the putative agent is effective to inhibit the metastatic cell (e, g, cancer metastasis). Also described are kits suitable for performing methods of the invention.

REFERENCES:
patent: 2008/0213258 (2008-09-01), Massague et al.
patent: WO 2006/074367 (2006-07-01), None
Drexler et al (Leukemia and Lymphoma, 1993, 9:1-25).
Zellner et al (Clin. Can. Res., 1998, 4:1797-17802).
Stein et al (Cancer Research, Apr. 2004, 64:2805-2816).
Norton and Massague (Nature Medicine, Aug. 2006, 12:875-878, IDS).
Hyoudou et al (Clinical Cancer Research, 2004, 10:7685-7691).
Hart (American Journal of Pathology, 1979, 97:587-600).
Minn, et al., “Genes that mediate breast cancer metastasis to lung”, Nature, 436, 2005, pp. 518-524.
Norton et al., “Is cancer a disease of self-seeding?”, Nature Medicine, 12(8), 2006, pp. 875-878.
Minn et al., “Lung metastasis genes couple breast tumor size and metastatic spread”, PNAS, 104(16), 2007, pp. 6740-5745.
Kim, et al., “Tumor Self-Seeding by Circulating Cancer Cells”, Cell 139, 2009, pp. 1315-1326 plus supplemental pages.
Leung et al., “Tumor Self-Seeding: Bidirectional Flow of Tumor Cells”, Cell, 139, 2009, pp. 1226-1228.
Hujanen et al., “Migration of Tumor Cells to Organ-derived Chemoattractants”, Cancer Research, 45, 1985, pp. 3517-3521.
Tanaka et al., “Chemokines in tumor progression and metastasis”, Cancer Sci, 96(6), 2005, pp. 317-322.

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