Assay for analysis of whole blood

Details Assay for analysis of whole blood Assay for analysis of whole blood

1983-10-28

1987-01-20

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving cholesterol

435 14, 435 25, 435 28, 435805, 436169, 436170, 436810, 422 56, 422 57, C12Q 160, C12Q 154, C12Q 126, C12Q 128

Patent

active

046379789

ABSTRACT:
Disclosed herein is an assay useful for the determination of an analyte in whole blood. In particular, this assay is useful for the quantitative determination of peroxide-generating analytes, such as glucose or cholesterol, in whole blood. This assay utilizes a multizone element consisting essentially of a support having thereon, in order and in fluid contact, a registration zone and a reagent/spreading zone. The reagent/spreading zone has a void volume and average pore size effective to accommodate whole blood, and contains an interactive composition necessary for the analysis. Such composition is capable of providing, upon interaction with the analyte, a dye which can be spectrophotometrically detected at a wavelength greater than about 600 nm.

REFERENCES:
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patent: 3992158 (1976-11-01), Przybylowicz et al.
patent: 4042335 (1977-08-01), Clement
patent: 4098574 (1978-07-01), Dappen
patent: 4144306 (1979-03-01), Figueras
patent: 4258001 (1981-03-01), Pierce et al.
patent: 4292272 (1981-09-01), Kitajima et al.
patent: 4312834 (1982-01-01), Vogel et al.
patent: 4384042 (1983-05-01), Miike et al.

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