Assay for an analyte on a solid porous support

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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2105003, 422101, 435174, 435301, 435 792, 436501, 436518, 436530, 436823, 436824, 530810, G01N 3353, G01N 33543, G01N 33544, B01D 3900

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active

049904426

ABSTRACT:
An assay for an analyte wherein sample is applied to a support capable of binding proteins by essentially only hydrogen bonding and fixed on the support. Analyte may be determined on the support by use of a suitable tracer. A preferred support is amphiphilic cellulose acetate. In an immunoassay, it is possible to determine analyte without use of a supported ("capture") antibody.

REFERENCES:
patent: 4193983 (1980-03-01), Ullman et al.
patent: 4391904 (1983-07-01), Litman et al.
patent: 4493815 (1985-01-01), Fernwood et al.
Maggio, Enzyme-Immunoassay, CRC Press, Inc., Boca Raton, Fl., 167-178 (1980).
Schleicher & Schuell brochure, Minifold, 1981.
Bio-Rad Catalog, 1984, pp. 167-168.
Hawkes, Methods in Enzymology, Academic Press, Inc., New York, May 1986, pp. 484-491.

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