Assay for 1,25-dihydroxy vitamin D receptor protein

Details Assay for 1,25-dihydroxy vitamin D receptor protein Assay for 1,25-dihydroxy vitamin D receptor protein

1989-08-01

1991-11-12

Wax,, Robert

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

Separation of immune complex from unbound antigen or antibody

436501, 436536, G01N 33536, G01N 33566

Patent

active

050647709

ABSTRACT:
An immunoassay assay for 1,25-dihydroxy vitamin D receptor protein (3) is disclosed. It uses two antibodies (1) (7), one labelled (2) and one anchored with biotin (6). The two antibodies bind to different epitopes (4) (5) of the receptor (3) provided, and the biotin (6) permits use of avidin sepharose (8) to separate labelled receptor protein for measurement.

REFERENCES:
patent: 4816417 (1989-03-01), DeLuca et al.
M. Sandgren et al., 183 Anal. Biochem. 57-63 (Nov. 1989) (not prior art).
P. Milde et al., 37 J. Hist. Cyto. 1609-1617 (Nov. 1989) (not prior art).
M. Dame et al., 25 Biochem. 4523-4534 (1986).
E. Pierce et al., 261 Arch. Biochem. Biophys. 241-249 (1988).
E. Pierce et al., 153 Anal. Biochem. 67-74 (1986).
M. Dame et al., 82 P.N.A.S. U.S.A. 7825-7829 (1985).
T. Brown et al., 85 P.N.S.A. U.S.A. 2454-2458 (1988).
W. Wecksler et al., 92 Anal. Biochem. 314-323 (1979).
R. Shepard et al., 182 J. Biochem. 55-69 (1979).
S. Dokoh et al., 221 J. Biochem. 129-136 (1984).
J. Gershoni et al., 131 Anal. Biochem. 1-15 (1983).
D. Williams et al., 13 Biochem. 5537-5542 (1974).
G. Leclercq et al., 46 Can. Res. 4233s-4236s (1986).
S. Brailly et al., 116 J. Endocr. 427-434 (1988).
L. Miles et al., 219 Nature 186-189 (1968).
D. Hullet, Ph.D. Thesis, University Of Wisconsin-Madison 180-204 (1984).
A. Bolton et al., 133 J. Biochem. 529-539 (1973).
J. Kohn et al., 107 Biochem. Biophys. Res. Com. 878-884 (1982).
U. Laemmli, 27 Nature 680-685 (1970).
M. Bradford, 72 Anal. Biochem. 248-254 (1976).
J. Pike, 43 Nutri. Reviews 161-168 (1985).
Undated advertisement by Serono Diagnostics showing their magnetic antibody immuno assay system (admitted prior art).
J. Napoli et al., 19 Biochem. 2515-2521 (1980).

Affiliated with

Also associated with

Rating

Assay for 1,25-dihydroxy vitamin D receptor protein does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Assay for 1,25-dihydroxy vitamin D receptor protein, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Assay for 1,25-dihydroxy vitamin D receptor protein will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1012823