Assay for 1,25-dihydroxy vitamin D in sample containing vitamin

Details Assay for 1,25-dihydroxy vitamin D in sample containing vitamin Assay for 1,25-dihydroxy vitamin D in sample containing vitamin

1987-08-14

1989-03-28

Marantz, Sidney

Chemistry: analytical and immunological testing

Biospecific ligand binding assay

436539, 436542, 436548, 436804, 436808, 436815, G01N 3353, G01N 33566, G01N 3360, G01N 3382

Patent

active

048164178

ABSTRACT:
An assay for 1,25-dihydroxy vitamin D is disclosed. One aspect of the invention involves adding pig receptor protein, radiolabeled, 1,25-dihydroxy vitamin D and biotinylated antibody capable of binding to the receptor to untreated blood serum. In performing a competitive binding assay, vitamin D transport protein, DBP, acts as a screen to minimize interference from related metabolites. A kit and an assay are disclosed.

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