Assay employing binding pair members on particles and on a filte

Details Assay employing binding pair members on particles and on a filte Assay employing binding pair members on particles and on a filte

1986-03-18

1990-10-02

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

436518, 436528, 436531, 436533, 436808, 435810, G01N 3353, G01N 33535, G01N 33543, G01N 33544

Patent

active

049606921

ABSTRACT:
An assay method and kit in which particles bearing a first reagent binding pair member (e.g., anti-digoxin antibody) react with a sample such that analyte binding pair member (e.g., digoxin) binds to the first reagent binding pair member. The reaction mixture is then passed through a filter or membrane or pore size sufficient to allow particles to pass through. A second reagent binding pair member (e.g., digoxin-albumin conjugate) is immobilized on the filter or membrane to trap preferentially either particles which have bound analyte binding pair members or particles which have not, leaving the other class of particles to pass through the filter for detection by resistive pulse techniques, by light absorbence or scattering, by enzymatic read-out (when the particles are enzyme-labeled) or otherwise.

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Pall Biotechnology Division, "Pall Biodyne.TM. Immounoaffinity Membrane" (Feb. 1985) Product Information Bulletin.
L. Blankenstein et al., "An Advanced Affinity Membrane for Immunodiagnostic Tests" Am Clin Prod Rev 33-41 (Nov. 1985).
Zuk et al., "Enzyme Immunochromatography" Clin. Chem. 31(7) pp. 1144-1150 (Nov. 1985).

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