Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure
Patent
1978-05-16
1980-11-25
Fagelson, Anna P.
Drug, bio-affecting and body treating compositions
Radionuclide or intended radionuclide containing; adjuvant...
Molecular bilayer structure
23230B, 250302, 424 1, 424 12, 424 13, 435 7, G01N 2100, G01N 3100, G01N 3114, G01N 3316
Patent
active
042358698
ABSTRACT:
Methods and compositions are provided for improved protein binding assays by preparing compositions having indirectly labeled ligands substantially free of label conjugated to materials other than the indirectly labeled ligand. The method for preparing the compositions involves employing a monovalent receptor to which the label is conjugated and combining the monovalent receptor labeled conjugate to the ligand, either in pure or impure form. The mixture is then segregated according to molecular weight and the ligand conjugated to the labeled receptor isolated. This conjugate may then be directly used as a reagent in a protein binding assay, where the assay mixture is substantially free of label other than labeled receptor bound to ligand.
The labels will be for the most part of relatively low molecular weight, while the receptor is preferably a Fab fragment. The ratio of receptor to ligand will be chosen so as to provide reasonable molecular weight distinctions between unbound ligand, unbound labeled receptor, ligand bound to labeled receptor, and other materials which may be in the mixture. Various techniques may be used for the separation.
The assays are performed in accordance with known methods employing a second receptor composition, which may be labeled or unlabeled.
REFERENCES:
patent: 3935074 (1976-01-01), Rubenstein
patent: 3996345 (1976-12-01), Ullman
patent: 3998943 (1976-12-01), Ullman
patent: 4104029 (1978-08-01), Maier
Ternynck et al., Ann. Immunol. (Inst. Pasteur), vol. 127 C 1976 pp. 197-208.
Weir (Ed.), Handbook of Exptl. Immunology, Blackwell Sci. Pub. London, 2nd ed., 1973, pp. 14.19-14.25.
Carrico, et al., Anal. Biochem. vol. 72, 1972 pp. 271-282, 283-292.
Forsum, J. of Immuno. Methods, vol. 2, 1972 pp. 183-195.
Fagelson Anna P.
Rowland Bertram I.
Syva Company
LandOfFree
Assay employing a labeled Fab-fragment ligand complex does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Assay employing a labeled Fab-fragment ligand complex, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Assay employing a labeled Fab-fragment ligand complex will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1129646