Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1989-03-08
1992-10-20
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 11, 435 25, 435 28, C12Q 160, C12Q 134, C12Q 126, C12Q 128
Patent
active
051569540
ABSTRACT:
An assay device and method for determining the concentration of an analyte. Analyte introduced by liquid absorption into the matrix is used to generate H2O2, via an analyte-specific oxidase, and the H.sub.2 O.sub.2 is utilized by peroxidase in the presence of peroxidase to convert a substrate reagent to a colored reaction product. A trapping compound in the matrix competes with the substrate reagent, in the presence of the peroxidase enzyme, to produce a silent reaction product, thus proportionately reducing the amount of signal reaction product generated by a given amount of analyte. Detection of a wider range of analyte concentrations is therefore possible.
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Hewett Gary E.
Mielke Steven T.
Cholestech Corporation
Dehlinger Peter J.
Reardon T. J.
Robinson Douglas W.
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