Assay device and method

Details Assay device and method Assay device and method

2004-12-29

2010-06-15

Yu, Melanie (Department: 1641)

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

C422S050000, C422S051000, C422S051000, C422S068100, C422S082050, C435S007100, C435S283100, C435S287100, C436S518000, C436S524000, C436S525000, C436S043000, C436S052000, C436S053000

Reexamination Certificate

active

07736890

ABSTRACT:
An assay method is described, which comprises the steps of immobilizing a binding partner (e.g., an antigen or antibody) for an analyte to be detected (e.g., an antibody or antigen) on a portion (140) of a surface (130) of a microfluidic chamber (120,122,124); passing a fluid sample over the surface and allowing the analyte to bind to the binding partner; allowing a metal colloid, e.g., a gold-conjugated antibody, to associate with the bound analyte; flowing a metal solution, e.g., a silver solution, over the surface such as to form an opaque metallic layer; and detecting the presence of said metallic layer, e.g., by visual inspection or by measuring light transmission through the layer, conductivity or resistance of the layer, or metal concentration in the metal solution after flowing the metal solution over the surface.

REFERENCES:
patent: 4517302 (1985-05-01), Saros
patent: 4687736 (1987-08-01), Newman et al.
patent: 4775636 (1988-10-01), Moeremans et al.
patent: 4931384 (1990-06-01), Layton et al.
patent: 4963498 (1990-10-01), Hillman et al.
patent: 5116734 (1992-05-01), Higgs et al.
patent: 5318621 (1994-06-01), Krulik et al.
patent: 5399497 (1995-03-01), Kumar et al.
patent: 5441896 (1995-08-01), Noppe et al.
patent: 5491098 (1996-02-01), Noppe et al.
patent: 5571410 (1996-11-01), Swedberg et al.
patent: 5595878 (1997-01-01), Sood et al.
patent: 5607863 (1997-03-01), Chandler
patent: 5637469 (1997-06-01), Wilding et al.
patent: 5637508 (1997-06-01), Kidwell et al.
patent: 5866345 (1999-02-01), Wilding et al.
patent: 5939252 (1999-08-01), Lennon et al.
patent: 5957579 (1999-09-01), Kopf-Sill et al.
patent: 6025202 (2000-02-01), Natan
patent: 6103199 (2000-08-01), Bjornson et al.
patent: 6146489 (2000-11-01), Wirth
patent: 6146589 (2000-11-01), Chandler
patent: 6186660 (2001-02-01), Kopf-Sill et al.
patent: 6214560 (2001-04-01), Yguerabide et al.
patent: 6241560 (2001-06-01), Furusawa et al.
patent: 6277489 (2001-08-01), Abbott et al.
patent: 6333200 (2001-12-01), Kaler et al.
patent: 6361958 (2002-03-01), Shieh et al.
patent: 6468657 (2002-10-01), Hou et al.
patent: 6479299 (2002-11-01), Parce et al.
patent: 6488872 (2002-12-01), Beebe et al.
patent: 6488894 (2002-12-01), Miethe et al.
patent: 6488896 (2002-12-01), Weigl et al.
patent: 6551841 (2003-04-01), Wilding et al.
patent: 6602669 (2003-08-01), Letsinger et al.
patent: 6613512 (2003-09-01), Kopf-Sill et al.
patent: 6709869 (2004-03-01), Mian et al.
patent: 6716620 (2004-04-01), Bashir et al.
patent: 6742661 (2004-06-01), Schulte et al.
patent: 6780584 (2004-08-01), Edman et al.
patent: 6794197 (2004-09-01), Indermuhle et al.
patent: 6818184 (2004-11-01), Fulwyler et al.
patent: 6967251 (2005-11-01), Haugland et al.
patent: 7110585 (2006-09-01), Cork et al.
patent: 7267948 (2007-09-01), Vo-Dinh
patent: 2001/0002315 (2001-05-01), Schultz et al.
patent: 2002/0019059 (2002-02-01), Chow et al.
patent: 2002/0142618 (2002-10-01), Parce et al.
patent: 2003/0064507 (2003-04-01), Gallagher et al.
patent: 2003/0118486 (2003-06-01), Zhou et al.
patent: 2003/0138969 (2003-07-01), Jakobsen et al.
patent: 2003/0139886 (2003-07-01), Bodzin et al.
patent: 2003/0207328 (2003-11-01), Yguerabide et al.
patent: 2003/0211488 (2003-11-01), Mirkin et al.
patent: 2003/0215865 (2003-11-01), Mayer et al.
patent: 2004/0014106 (2004-01-01), Patno et al.
patent: 2004/0077074 (2004-04-01), Ackley et al.
patent: 2004/0086897 (2004-05-01), Mirkin et al.
patent: 2004/0146917 (2004-07-01), Cork et al.
patent: 2004/0191921 (2004-09-01), Farquharson et al.
patent: 2005/0059030 (2005-03-01), Bao et al.
patent: 2005/0130174 (2005-06-01), Bao et al.
patent: 2005/0141843 (2005-06-01), Warden et al.
patent: 2005/0282288 (2005-12-01), Farquharson et al.
patent: 2006/0014161 (2006-01-01), Landt et al.
patent: 2006/0014172 (2006-01-01), Muller et al.
patent: 2006/0084069 (2006-04-01), Chan et al.
patent: 2006/0147927 (2006-07-01), Geddes et al.
patent: 2007/0041624 (2007-02-01), Cork et al.
patent: 2007/0148665 (2007-06-01), Cork et al.
patent: 2007/0298433 (2007-12-01), Sia et al.
patent: 461174 (1991-12-01), None
patent: 481020 (1992-04-01), None
patent: 0643307 (1995-03-01), None
patent: 928420 (1999-07-01), None
patent: 1054259 (2000-11-01), None
patent: 1439392 (2005-07-01), None
patent: 1740951 (2007-01-01), None
patent: 1798556 (2007-06-01), None
patent: 1912067 (2008-04-01), None
patent: 1912068 (2008-04-01), None
patent: WO 91/01003 (1991-01-01), None
patent: WO 02/14869 (2002-02-01), None
patent: WO 03/054513 (2003-07-01), None
patent: WO 2004/087951 (2004-10-01), None
patent: WO 2004/087951 (2004-10-01), None
patent: WO 2005047864 (2005-05-01), None
patent: WO 2005123966 (2005-12-01), None
patent: WO 2006022495 (2006-03-01), None
patent: WO 2006/065762 (2006-06-01), None
patent: WO 2006/116037 (2006-11-01), None
patent: WO 2006/122222 (2006-11-01), None
patent: WO 2007/047455 (2007-04-01), None
patent: WO 2007/135591 (2007-11-01), None
patent: WO 2007/135593 (2007-11-01), None
patent: WO 2008/013813 (2008-01-01), None
Ahn, C., et al., “Disposable Smart Lab on a Chip for Point-of-Care Clinical Diagnostics,”Proceedings of the IEEE, vol. 92, No. 1, pp. 154-173 (2004).
Andersson, et al., “Micromachined flow-through filter-chamber for chemical reactions on beads,”Sensors and Actuators,vol. B67, pp. 203-208 (2000).
Darion, et al., “Chemical sensing using an integrated microfluidic system based on the Berthelot reaction,”Sensors and Actuators,vol. B76, pp. 235-243 (2001).
Dodge, et al., “Electrokinetically Driven Microfluidic Chips with Surface-Modified Chambers for Heterogeneous Immunoassays,”Anal. Chem.,vol. 73, pp. 340-3409 (2001).
Juncker, et al., “Autonomous Microfluidic Capillary Systems,”Anal. Chem,vol. 74, pp. 6139-6144 (2002).
Moorthy, et al., “Microfluidic tectonics platform: A colorimetric, disposable botulinum toxin enzyme-linked immunosorbent assay system,”Electrophorsis,vol. 25, pp. 1705-1713 (2004).
Obeid, et al., “Microfabricated Device for DNA and RNA Amplification by Continuous-Flow Polymerase Chain Reaction and Reverse Transcription-Polymerase Chain Reaction with Cycle Number Selection,”Anal. Chem.,vol. 75, pp. 288-295 (2003).
Sia, S., et al., “An Integrated Approach to a Portable and Low-Cost Immunoassay for Resource-Poor Settings,”Angew. Chem. Int. Ed.,vol. 43, pp. 498-502 (2004).
Sia, S., et al., “Microfluidic devices fabricated in poly(dimethlysiloxane) for biological studies,”Electrophoresis,vol. 24, pp. 3563-3576 (Nov. 2003).
Weigle, et al., “Lab-on-a-chip for drug development,”Advanced Drug Delivery Reviews,vol. 55, pp. 349-377 (2003).
Proceedings of uTAS 2004, 8th International Conference on Miniaturized Systems in Chemistry and Life Sciences, Sep. 26-30, Malmo, Sweden, Edited by Thomas Laurell, Johan Nilsson, Klavs Jensen, D. Jed Harrison, Jorg P. Kutter, The Royal Society of Chemistry, pp. 1-135, 2004.

Affiliated with

Also associated with

Rating

Assay device and method does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Assay device and method, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Assay device and method will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4165006