Assay by osmotically induced separation and concentration of...

Chemistry: analytical and immunological testing – Involving immune complex formed in liquid phase

Reexamination Certificate

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C422S068100, C422S071000, C422S082000, C422S082010, C422S082050, C422S105000, C422S105000, C422S105000, C422S105000, C435S004000, C435S007100, C435S007900, C435S287200, C435S288200, C435S288500, C435S288700, C435S968000, C436S501000, C436S504000, C436S542000, C436S149000, C436S164000

Reexamination Certificate

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07892857

ABSTRACT:
The invention relates to an assay method and a fluid microsystem for carrying out said assay method, in particular, for carrying out miniaturized affinity tests in a micro-array format. According to said invention, a liquid phase comprising at least one type of detectable high-molecular weight soluble substance, for example a labeled reaction partner or product of an affinity reaction is displaced by the hydraulic effect of a high-molecular weight osmotic agent on an ultrafiltration membrane towards a miniaturized measuring chamber defined thereby. The fraction of the detectable high-molecular weight substance(s) is concentrated in the measuring chamber, while the dissolved low-molecular weight components are removed with the solvent through the membrane pores, thereby making it possible to attain a high detection sensitivity.

REFERENCES:
patent: 5045193 (1991-09-01), Pinon
patent: 5770086 (1998-06-01), Indriksons
patent: 5894061 (1999-04-01), Ladouceur
patent: 2002/0086309 (2002-07-01), Benn et al.
patent: 2002/0106787 (2002-08-01), Benn
patent: 19729492 (1997-10-01), None
patent: 10134860 (2003-02-01), None
patent: 10311622 (2004-10-01), None
patent: WO-02/15949 (2002-02-01), None

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