Assay by enzyme-catalyzed isotopic exchange

Details Assay by enzyme-catalyzed isotopic exchange Assay by enzyme-catalyzed isotopic exchange

1992-03-26

1993-12-21

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 772, 435 79, 435 15, 435 26, 435189, 435191, 435810, 435814, 435968, 435975, 436504, 436542, 436545, 436804, 424 11, C12Q 100, G01N 33567

Patent

active

052720543

ABSTRACT:
A method of assay for isotopically exchangeable analytes is disclosed. Analytes are labeled by enzymatic exchange of a hydrogen atom of the analyte and a deuterium or tritium atom. Preferably, analytes are labeled by reaction with an oxidant, a reducing agent which contains a deuterium or tritium atom, and an enzyme capable of catalyzing the reversible exchange of a hydrogen atom between the analyte, the oxidant, and the reducing agent. Kits for conveniently performing the assay methods are also disclosed.

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