Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1993-08-17
1995-11-07
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 692, 435 19, 435 20, 435 11, 560152, 514103, 514 86, 514126, C12Q 134, C12P 2106, C07C32100, A01N 5736
Patent
active
054647541
ABSTRACT:
A non-radioactive, spectrophotometric, microtiter plate assay for human cystolic phospholipase A.sub.2 (cPLA.sub.2) is described. The assay utilizes a novel synthetic thiol-phospholipid analog as a substrate. In one embodiment, the substrate is a phosphatidylcholine derivative with an arachidonoylthioester in the sn-2 position and an alkenyl-ether or alkenyl-ether in the sn-1 position. The alkyl-ether and the alkenyl-ether in the sn-1 position of the substrate ensures that the assay will only measure cPLA.sub.2 activity and will not be complicated by metabolism of the lysophospholipid product by the enzyme's and lysophospholypase activity.
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Dennis Edward A.
Reynolds Laure J.
Yu Lin
Brunelle Jan P.
Dreger Walter H.
Leary Louise N.
The Regents of the University of California
Wityshyn Michael G.
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