Chemistry: analytical and immunological testing – Composition for standardization – calibration – simulation,... – Lipid – cholesterol – or triglyceride standard or control
Reexamination Certificate
2005-08-23
2009-12-29
Gakh, Yelena G (Department: 1797)
Chemistry: analytical and immunological testing
Composition for standardization, calibration, simulation,...
Lipid, cholesterol, or triglyceride standard or control
C436S071000, C436S060000, C436S174000, C436S177000
Reexamination Certificate
active
07638336
ABSTRACT:
The present invention relates to an assay for determining the levels of sterols, stanols, steryl esters, fatty acid derivatives and combinations thereof in a starch-containing food product. The assay is particularly useful in supporting product health and/or nutritional claims in manufacturing products intended for human or animal consumption. The present invention describes a method for extracting sterols related compounds and uses as an internal standard a steryl ester, preferably cholesteryl oleate. By using the present extraction technique the process enables the recovery of substantially all of the sterol related compound in the sample.
REFERENCES:
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Gordon et al. “Development of steryl ester analysis for the detection of admixtures of vegetable oils”, J. Am. Oil Chem., 1997, v. 74, No. 5, pp. 505-510.
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Ahamed, et al., Lipopolysaccharide with 2,3-diamino-2,3-dideoxyglucose containing lipid A inRhodopseudomonas sulfoviridis, Elsevier Biomedical Press, FEMS Microbiology Letters 14 (1982) 27-30.
Krekhova et al., “Fractional determination of cholesterol esters in blood and tissues by thin-layer chromatography,” Voprosy Meditsinskoi Khimii (1971), 17(1), 93-8, Abstract only.
Abidi, “Chromatographic analysis of plant sterols in foods and vegetable oils,” Elsevier Science, Journal of Chromatography A. 935 (2001) 173-201.
Rivabene, et al., “Redox-Dependent Modulation of Lipid Synthesis Induced by Oleic Acid in the Human Intestinal Epithelial Cell Line Caco-2,” Exp. Biology and Medicine, vol. 226(3):191-198 (2001).
Devries Jonathan W.
Lewandowski Daniel J.
Plank David W.
Bjorkman Dale
Gakh Yelena G
General Mills Inc.
O'Toole John A.
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