Chemistry: analytical and immunological testing – Composition for standardization – calibration – simulation,... – Lipid – cholesterol – or triglyceride standard or control
Reexamination Certificate
2005-09-06
2005-09-06
Gakh, Yelena G. (Department: 1743)
Chemistry: analytical and immunological testing
Composition for standardization, calibration, simulation,...
Lipid, cholesterol, or triglyceride standard or control
C436S071000, C436S060000, C436S174000, C436S177000
Reexamination Certificate
active
06939713
ABSTRACT:
The present invention relates to an assay for determining the levels of sterols, stanols, steryl esters, fatty acid derivatives and combinations thereof in a starch-containing food product. The assay is particularly useful in supporting product health and/or nutritional claims in manufacturing products intended for human or animal consumption. The present invention describes a method for extracting sterols related compounds and uses as an internal standard a steryl ester, preferably cholesterol oleate. By using the present extraction technique the process enables the recovery of substantially all of the sterol related compound in the sample.
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Gordon et al. “Development of steryl ester analysis for the detection of admixtures of vegetable oils”, J. Am. Oil Chem., 1997, v. 74, No. 5, pp. 505-510.
Rivabene et al. “Redox-dependent modulation of lipid synthesis induced by oleic acid in the human intestinal epithelial cell LineCaco-2”, Exp. Biol. Med., 2001, v. 226, pp. 191-198.
Lewandowski Daniel J.
Plank David W.
Bjorkman Dale A.
Gakh Yelena G.
General Mills Inc.
Hornilla Arlene L.
Taylor Douglas J.
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