Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2007-02-23
2010-11-23
Wessendorf, Teresa D. (Department: 1639)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S007200, C435S007100, C435S006120, C435S004000
Reexamination Certificate
active
07838256
ABSTRACT:
The present invention relates to high-throughput cell-based assays for real-time monitoring of multi-resistant drug protein activity. The present invention is an improvement over existing assays in that in addition to a fluorescent drug efflux probe as an indicator of MDR protein activity, the instant assays provide an o-tolidine-based dye for quenching extracellular fluorescence of the probe.
REFERENCES:
patent: 5766875 (1998-06-01), Hafeman et al.
patent: 6277655 (2001-08-01), Sarkadi et al.
patent: 6391656 (2002-05-01), Sarkadi et al.
patent: 2003/0044316 (2003-03-01), Hirai et al.
patent: 2005/0130236 (2005-06-01), Goldman
patent: 96/06945 (1996-03-01), None
Homolya, L., et. al., “Fluorescent cellular indicators are extruded by the multidrug resistance protein,” J. Biol. Chem., 268(29):21493-96, (Oct. 15, 1993).
Hollo, Z., et al., “Calcein accumulation as a fluorometric functional assay of the multidrug transporter,” Biochim. Biophys. Acta., 1191(2):384-8, (1994).
Zhang, Y., et al., “In vitro and in vivo models for assessing drug efflux transporter activity,” Adv. Drug Del. Rev., 55:31-51, (2003).
Brezden, C.B., et al., “Constitutive expression of P-glycoprotein as a determinant of loading with fluorescent calcium probes,” Cytometry, 17:343-348, (1994).
Feller, N., et al., “ATP-dependent efflux of calcein by the multidrug resistance protein (MRP): no inhibition by intracellular glutathione depletion,” FEBS Lett., 368:385-388 (1995).
Goodfellow, H.R., et al., “Protein kinase C-mediated phosphorylation does not regulate drug transport by the human multidrug resistance P-glycoprotein,” J. Biol. Chem., 271(23):13668-74, (Jun. 7, 1996).
Hollo, Z., et al., “Transport properties of the multidrug resistance-associated protein (MRP) in human tumour cells,” FEBS Lett., 383:99-104, (1996).
Essodaigui, M., et al., “Kinetic analysis of calcein and calcein—acetoxymethylester efflux mediated by the multidrug resistance protein and P-glycoprotein,” Biochemistry, 37(8):2243-50, (1998).
Tang-Wai, D.F., et al., “Human (MDR1) and mouse (mdr1,mdr3) P-glycoproteins can be distinguished by their respective drug resistance profiles and sensitivity to modulators,” Biochemistry, 34(1):32-39, (1995).
Millot, J-M., et al., “Scanning microspectrofluorometry of rhodamine 123 in multidrug-resistant cells,” Cytometry, 17:50-58, (1994).
Nare, B., et al., “Characterization of rhodamine 123 binding to P-glycoprotein in human multidrug-resistant cells,” Mol. Pharmacol., 45:1145-52 , (1994).
Altenberg, G.A., et al., “Unidirectional fluxes of rhodamine 123 in multidrug-resistant cells: evidence against direct drug extrusion from the plasma membrane,” Proc. Natl. Acad. Sci. USA, 91:4654-4657, (May 1994).
Van Amersfoort, E.S., et al.,“Evaluation of a flow cytometric fluorescence quenching assay of phagocytosis of sensitized sheep erythrocytes by polymorphonuclear leukocytes,” Cytometry, 17(4):294-301, (1994).
Beyersman, D., et al., “The genetic toxicology of cobalt,” Toxicol. Appl. Pharmacol., 115(1):137-45, (1992).
Bachmeier, C.J., et al., “A fluorometric screening assay for drug efflux transporter activity in the blood-brain barrier,” Pharmaceutical Research, 22(1):115-121, (Jan. 2005).
Bachmeier Corbin J.
Lomneth Richard B.
Miller Donald W.
Board of Regents of the University of Nebraska
Dann Dorfman Herrell and Skillman, P.C.
Hagan Patrick J.
Wessendorf Teresa D.
LandOfFree
Assay and kit for drug efflux transporter activity does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Assay and kit for drug efflux transporter activity, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Assay and kit for drug efflux transporter activity will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4194262