Assay and kit for determining interferon

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

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435 7, 435810, 436531, 436804, 436808, 436828, G01N 3353, G01N 33545, G01N 3360

Patent

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046222928

ABSTRACT:
An assay and kit for the detection and quantitative determination of interferon. The assay is based on the exposure of certain cells to a solution containing the interferon which is to be determined, infecting the cells with a certain virus, incubating for a predetermined period of time, lysing the infected cultures and determining the virus protein. The measurement of the virus proteins can be effected by ELISA or radioimmunoassay.

REFERENCES:
Julkunen, I. et al., J. Virol. Methods, 5(2), 85-91 (1982).
Lyons, S. F. et al., J. Virol. Methods, 5(2), 93-100 (1982).

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