Assay

Details Assay Assay

2008-03-18

2008-03-18

Siew, Jeffrey (Department: 1645)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C435S002000, C435S004000, C435S007100, C435S007400, C435S007500, C435S007600, C435S007910, C435S007920, C435S007930, C435S007940, C436S043000, C436S066000, C436S086000, C436S164000, C436S174000, C436S501000, C436S512000, C436S519000, C436S532000, C436S536000, C436S538000, C436S540000

Reexamination Certificate

active

10897433

ABSTRACT:
The present invention relates to assay methods for the determination of cobalamin or vitamin B12in a body fluid and in particular to assay methods for the metabolically active pool of cobalamin, comprising contacting a cell free sample of a body fluid with an immobilised or immobilizable specific binding ligand for TC II or holo TC II, separating a ligand bound fraction from a non-ligand bound fraction and measuring the holo-TC II or TC-II bound cobalamin content therein.

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Nexoe, Ebba, “Characterization of the cobalamins attached to transcobalamin I and transcobalamin II in human plasma”, 1977, XP002127542.
Rothenberg S.P., Quadros E.V., “Transcobalamin II and the membrane receptor of the transcobalamin II-cobalamin complex”, 1995, XP002127541.
Kuemmerle et al. 1992. Automated Assay of Vitamin B12 by the Abbott IMx (TM) Analyzer. Clin Chem. 38(10):2073-2077.
McLean et al. 1997. Antibodies to Transcobalamin II Block In Vitro Proliferation of Leukemic Cells. Blood. 89(1):235-242.
Quadros et al. 1996. Characterization of Monoclonal Antibodies to Epitopes of Human Transcobalamin II. Biochem. Biophy. Res. Comm. 222:149-154.
Wickramasinghe et al. 1993. Correlations between holo-transcobalamin II, holo-haptocorrin, and total B12 in serum samples from healthy subjects and patients. J. Clin. Path. 46:537-539.
Nexoe, Ebba, “Characterization of the cobalamins attached to transcobalamin I and transcobalamin II in human plasma”, 1977, XP002127542, Abstract Only.
Rothenberg S.P., Quadros E.V., “Transcobalamin II and the membrane receptor of the transcobalamin II-cobalamin complex”, 1995, XP002127541, Abstract Only.

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