Asporogenic mutant of Bacillus subtilis useful as a host in a ho

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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4351721, 435839, 935 74, 935 58, C12N 120, C12N 1500, C12R 125

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044502362

ABSTRACT:
A biologically pure strain of asporogenous B. subtilis DE101 (ATCC 39,095) is provided. This strain, which shows a low frequency of reversion to spore formers, is suitable for a host providing a high level of biological containment in a host-vector system for use in recombinant DNA methodology.

REFERENCES:
patent: 4237224 (1980-12-01), Cohen et al.
patent: 4302544 (1981-11-01), Young et al.
Ellis, D. M. and Dean, D. H., Recombinant DNA Technical Bulletin, vol. 4, No. 1, Mar. 1981.
Burke, W. F. and Le, H. T., Recombinant DNA Technical Bulletin, vol. 3, No. 1, Dec. 1980.
Dedonder, et al, Applied and Environmental Microbiology, 33, 989-993, (1977).
Farmer and Rothman, J. Bacteriology, 89, 262-263, (1965).

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