Aspergillus niger transformation system

Details Aspergillus niger transformation system Aspergillus niger transformation system

1984-12-05

1989-12-05

Martinell, James

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 691, 4351721, 435254, 435320, 935 55, 935 56, 935 68, C12N 1500, C12N 114, C12P 2100

Patent

active

048852490

ABSTRACT:
Transformants of Aspergillus niger and related Aspergilli, containing foreign DNA conferring modified properties of expression thereon, are prepared by use of a DNA vector which contains a selectable marker which is capable of incorporation into the DNA of the host A. niger cells, but which is not to be found in the A. niger cells prior to this transformation. The vector also contains other foreign DNA to be incorporated into the A. niger, for modified expression. The process suitably uses mutants of A. niger as hosts, the mutants lacking the selectable marker as compared with wild type A. niger, and conducts the transformation on spheroplasts of A. niger.

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